J09:0010a	-	YB	<minbrk>	-	[Oh.Oh]
J09:0010b	-	AT1	A	a	[O[S[Ns:S.
J09:0010c	-	NN1n	variety	variety	.
J09:0010d	-	IO	of	of	[Po.
J09:0010e	-	NN2	techniques	technique	.Po]Ns:S]
J09:0010f	-	VH0	have	have	[Vfp.
J09:0010g	-	VBN	been	be	.
J09:0010h	-	VVNv	directed	direct	.Vfp]
J09:0010i	-	II	toward	toward	[P:q.
J09:0010j	-	AT	the	the	[N.
J09:0010k	-	NN1u	isolation	isolation	[NN1u&.
J09:0020a	-	CC	and	and	[NN1n+.
J09:0020b	-	NN1n	study	study	.NN1n+]NN1u&]
J09:0020c	-	IO	of	of	[Po.
J09:0020d	-	NN1n	blood	blood	[Np[Ns.
J09:0020e	-	NNJ1c	group	group	.Ns]
J09:0020f	-	NN2	antibodies	antibody	.Np]Po]N]P:q]S]
J09:0020g	-	YF	+.	-	.
J09:0020h	-	DD2i	These	these	[S[Dp:s.Dp:s]
J09:0020i	-	VV0t	include	include	[V.V]
J09:0020j	-	JJ	low	low	[N:o[Ns.
J09:0020k	-	YH	+<hyphen>	-	.
J09:0020m	-	NN1n	+temperature	temperature	.Ns]
J09:0030a	-	NN1u	ethanol	ethanol	.
J09:0030b	-	YPL	(	-	.
J09:0030c	-	NP1s	+Cohn	Cohn	[Nns.Nns]
J09:0030d	-	YPR	+)	-	.
J09:0030e	-	NN1u	fractionation	fractionation	.
J09:0030f	-	YC	+,	-	.
J09:0030g	-	NN1u	electrophoresis	electrophoresis	[Ns-.Ns-]
J09:0030h	-	YC	+,	-	.
J09:0030i	-	NN1u	ultracentrifugation	ultracentrifugation	[Ns-.Ns-]
J09:0030j	-	CC	and	and	[Ns+.
J09:0040a	-	NN1c	column	column	.
J09:0040b	-	NN1u	chromatography	chromatography	.
J09:0040c	-	II	on	on	[P.
J09:0040d	-	NN1c	ion	ion	[Np[Ns.
J09:0040e	-	NN1n	exchange	exchange	.Ns]
J09:0040f	-	NN2	celluloses	cellulose	.Np]P]Ns+]N:o]S]
J09:0040g	-	YF	+.	-	.
J09:0040h	-	NN2	Modifications	modification	[S[Np:S.
J09:0040i	-	IO	of	of	[Po.
J09:0050a	-	AT	the	the	[Ns.
J09:0050b	-	MDt	last	last	.
J09:0050c	-	NN1n	technique	technique	.Ns]Po]Np:S]
J09:0050d	-	VH0	have	have	[Vfp.
J09:0050e	-	VBN	been	be	.
J09:0050f	-	VVNv	applied	apply	.Vfp]
J09:0050g	-	IIb	by	by	[Pb:a.
J09:0050h	-	DA2q	several	several	[Np.
J09:0050i	-	NN2	groups	group	.
J09:0050j	-	IO	of	of	[Po.
J09:0050k	-	NN2	investigators	investigator	.Po]Np]Pb:a]S]
J09:0050m	-	YF	+.	-	.
J09:0060a	-	NP1s	Abelson	Abelson	[S[Nn:s[NP1s&.
J09:0060b	-	CC	and	and	[NP1s+.
J09:0060c	-	NP1s	Rawson	Rawson	.NP1s+]NP1s&]Nn:s]
J09:0060d	-	YC	+,	-	.
J09:0060e	-	VVGt	using	use	[Tg:h[Vg.Vg]
J09:0060f	-	AT1	a	a	[Ns:o.
J09:0060g	-	JB	stepwise	stepwise	[Ns.
J09:0060h	-	NN1n	elution	elution	.Ns]
J09:0060i	-	NN1c	scheme	scheme	.Ns:o]Tg:h]
J09:0060j	-	YC	+,	-	.
J09:0060k	-	VVDt	fractionated	fractionate	[Vd.Vd]
J09:0070a	-	JJ	whole	whole	[Np:o101.
J09:0070b	-	NN2	sera	serum	.
J09:0070c	-	YG	-	-	[Tg[s101.s101]
J09:0070d	-	VVGt	containing	contain	[Vg.Vg]
J09:0070e	S	FOs	ABO	-	[Np:o[FOs&.
J09:0070f	-	CC	and	and	[FWs+.
J09:0070g	S	FWs	Rh	-	.FWs+]FOs&]
J09:0070h	-	NN2	antibodies	antibody	.Np:o]Tg]Np:o101]
J09:0070i	-	II	on	on	[P:p.
J09:0070j	-	NN1u	diethylaminoethyl	diethylaminoethyl	[N[Ns.
J09:0080a	S	FOc	DEAE	-	.Ns]
J09:0080b	-	NN1u	cellulose	cellulose	.
J09:0080c	-	CC	and	and	[Ns+.
J09:0080d	-	NN1u	carboxymethyl	carboxymethyl	.
J09:0080e	-	NN1u	cellulose	cellulose	.Ns+]N]P:p]S]
J09:0080f	-	YF	+.	-	.
J09:0080g	-	NP1s	Speer	Speer	[S[Nn:s[NP1s&.
J09:0090a	-	CC	and	and	[NN2+.
J09:0090b	-	NN2	coworkers	co<hyphen>worker	.NN2+]NP1s&]Nn:s]
J09:0090c	-	YC	+,	-	.
J09:0090d	-	II	in	in	[P:p.
J09:0090e	-	AT1	a	a	[Ns.
J09:0090f	-	JJ	similar	similar	.
J09:0090g	-	NN1n	study	study	.
J09:0090h	-	IO	of	of	[Po.
J09:0090i	-	NN1n	blood	blood	[Np[Ns.
J09:0090j	-	NNJ1c	group	group	.Ns]
J09:0090k	-	NN2	antibodies	antibody	.
J09:0090m	-	IO	of	of	[Po.
J09:0090n	-	JJ	whole	whole	[Np.
J09:0100a	-	NN2	sera	serum	.Np]Po]Np]Po]Ns]P:p]
J09:0100b	-	YC	+,	-	.
J09:0100c	-	VVDt	used	use	[Vd.Vd]
J09:0100d	-	AT1	a	a	[Ns:o.
J09:0100e	-	NNc	series	series	.
J09:0100f	-	IO	of	of	[Po.
J09:0100g	-	NN2	gradients	gradient	.Po]Ns:o]
J09:0100h	-	IF	for	for	[P:c.
J09:0100i	-	NN1n	elution	elution	[Ns.
J09:0100j	-	II	from	from	[P.
J09:0100k	S	FOc	DEAE	-	[Ns.
J09:0100m	-	YH	+<hyphen>	-	.
J09:0100n	-	NN1u	+cellulose	cellulose	.Ns]P]Ns]P:c]S]
J09:0100p	-	YF	+.	-	.
J09:0110a	-	NP1s	Fahey	Fahey	[S[Nn:s[NP1s&.
J09:0110b	-	CC	and	and	[NP1s+.
J09:0110c	-	NP1s	Morrison	Morrison	.NP1s+]NP1s&]Nn:s]
J09:0110d	-	VVDt	used	use	[Vd.Vd]
J09:0110e	-	AT1	a	a	[Ns:o.
J09:0110f	-	JJ	single	single	[JJ&.
J09:0110g	-	YC	+,	-	.
J09:0110h	-	JJ	continuous	continuous	[JJ-.JJ-]JJ&]
J09:0110i	-	NN1c	gradient	gradient	.
J09:0110j	-	II	at	at	[P.
J09:0110k	-	JJ	constant	constant	[Nu.
J09:0120a	S	NNUb	pH	-	.Nu]P]Ns:o]
J09:0120b	-	IF	for	for	[P:c.
J09:0120c	-	AT	the	the	[Ns.
J09:0120d	-	NN1u	fractionation	fractionation	.
J09:0120e	-	IO	of	of	[Po.
J09:0120f	-	FB	anti	anti<hyphen>	[Np[J.
J09:0120g	-	YH	+<hyphen>	-	.
J09:0120h	S	ZZ1	+A	-	.
J09:0120i	-	CC	and	and	[J+.
J09:0120j	-	FB	anti	anti<hyphen>	.
J09:0120k	-	YH	+<hyphen>	-	.
J09:0120m	S	ZZ1	+B	-	.J+]J]
J09:0120n	-	NN2	agglutinins	agglutinin	.Np]Po]
J09:0130a	-	II	from	from	[P.
J09:0130b	-	JJ	preisolated	pre<hyphen>isolated	[Np.
J09:0130c	S	ZZ1	<ggr>	-	[Ns.
J09:0130d	-	YH	+<hyphen>	-	.
J09:0130e	-	NN1u	+globulin	globulin	.Ns]
J09:0130f	-	NN2	samples	sample	.Np]P]Ns]P:c]S]
J09:0130g	-	YF	+.	-	.O]
J09:0130h	-	YB	<minbrk>	-	[Oh.Oh]
J09:0130i	-	II	In	in	[O[S[P:p.
J09:0130j	-	AT	the	the	[Ns.
J09:0140a	-	JJ	present	present	.
J09:0140b	-	NN1n	work	work	.Ns]P:p]
J09:0140c	-	JJ	whole	whole	[Np:S.
J09:0140d	-	NN2	sera	serum	.Np:S]
J09:0140e	-	VH0	have	have	[Vfp.
J09:0140f	-	VBN	been	be	.
J09:0140g	-	VVNt	fractionated	fractionate	.Vfp]
J09:0140h	-	IIb	by	by	[Pb:h.
J09:0140i	-	NN1u	chromatography	chromatography	[Ns:103.
J09:0140j	-	II	on	on	[P.
J09:0145a	S	FOc	DEAE	-	[Ns.
J09:0145b	-	YH	+<hyphen>	-	.
J09:0145c	-	NN1u	+cellulose	cellulose	.Ns]P]
J09:0145d	-	YG	-	-	[Tg[s103.s103]
J09:0150a	-	VVGt	using	use	[Vg.Vg]
J09:0150b	-	JJ	single	single	[Np:o105.
J09:0150c	-	NN2	gradients	gradient	.
J09:0150d	-	JJ	similar	similar	[Jh.
J09:0150e	-	IIt	to	to	[P.
J09:0150f	-	DD2a	those	those	[Dp:105.
J09:0150g	-	YG	-	-	[Tn[S105.S105]
J09:0150h	-	VVNt	described	describe	[Vn.Vn]
J09:0160a	-	IIb	by	by	[Pb:a.
J09:0160b	-	NP1s	Sober	Sober	[Nn[NP1s&.
J09:0160c	-	CC	and	and	[NP1s+.
J09:0160d	-	NP1s	Peterson	Peterson	.NP1s+]NP1s&]Nn]Pb:a]Tn]Dp:105]P]Jh]Np:o105]Tg]Ns:103]Pb:h]
J09:0160e	-	YC	+,	-	.
J09:0160f	-	CC	and	and	[S+.
J09:0160g	-	JJ	certain	certain	[Np:S.
J09:0160h	-	JJ	chemical	chemical	[JJ&.
J09:0160i	-	CC	and	and	[JJ+.
J09:0160j	-	JJ	serological	serological	.JJ+]JJ&]
J09:0160k	-	NN2	properties	property	.
J09:0170a	-	IO	of	of	[Po.
J09:0170b	-	AT	the	the	[Np:107.
J09:0170c	-	NN2	fractions	fraction	.
J09:0170d	-	YG	-	-	[Tg[s107.s107]
J09:0170e	-	VVGt	containing	contain	[Vg.Vg]
J09:0170f	-	NN2	antibodies	antibody	[Np:o.
J09:0170g	-	IO	of	of	[Po.
J09:0170h	-	AT	the	the	[Np.
J09:0170i	S	FOs	ABO	-	[FOs&.
J09:0170j	-	CC	and	and	[FWs+.
J09:0170k	S	FWs	Rh	-	.FWs+]FOs&]
J09:0180a	-	NN2	systems	system	.Np]Po]Np:o]Tg]Np:107]Po]Np:S]
J09:0180b	-	VH0	have	have	[Vfp.
J09:0180c	-	VBN	been	be	.
J09:0180d	-	VVNt	described	describe	.Vfp]S+]S]
J09:0180e	-	YF	+.	-	.O]
J09:0180f	-	YBL	<bmajhd>	-	[Oh.
J09:0180g	-	NN2	MATERIALS	material	[Np[NN2&.
J09:0180h	-	CC	AND	and	[NN2+.
J09:0180i	-	NN2	METHODS	method	.NN2+]NN2&]Np]
J09:0180j	-	YBR	<emajhd>	-	.Oh]
J09:0180k	-	YBL	<bminhd>	-	[Oh.
J09:0180m	-	NN2	SAMPLES	sample	.
J09:0180n	-	YF	+.	-	.
J09:0180p	-	YBR	<eminhd>	-	.Oh]
J09:0190a	-	NN1u	Serum	serum	[O[S[Np:S.
J09:0190b	-	NN2	samples	sample	.Np:S]
J09:0190c	-	VBDR	were	be	[Vwp.
J09:0190d	-	VVNv	obtained	obtain	.Vwp]
J09:0190e	-	II	from	from	[P:q.
J09:0190f	-	JJ	normal	normal	[Np.
J09:0190g	-	NNJ1c	group	group	[Nn.
J09:0190h	S	ZZ1	A	-	.
J09:0190i	-	YC	+,	-	.
J09:0190j	-	NNJ1c	group	group	[Nns-.
J09:0190k	S	ZZ1	B	-	.Nns-]
J09:0200a	-	CC	and	and	[Nns+.
J09:0200b	-	NNJ1c	group	group	.
J09:0200c	S	ZZ1	O	-	.Nns+]Nn]
J09:0200d	-	NN2	donors	donor	.Np]P:q]S]
J09:0200e	-	YF	+.	-	.
J09:0200f	-	MC	Three	three	[S[M:S.
J09:0200g	-	IO	of	of	[Po.
J09:0200h	-	AT	the	the	[Np:109.
J09:0200i	-	FB	anti	anti<hyphen>	[P.
J09:0200j	-	YH	+<hyphen>	-	.
J09:0200k	S	FWs	+Rh	-	[Nn.Nn]P]
J09:0200m	-	NN2	sera	serum	.
J09:0200n	-	YG	-	-	[Tn[S109.S109]
J09:0200p	-	VVNt	used	use	[Vn.Vn]Tn]Np:109]Po]M:S]
J09:0200q	-	VBDR	were	be	[Vwp.
J09:0200r	-	VVNv	taken	take	.Vwp]
J09:0210a	-	II	from	from	[P:q.
J09:0210b	-	RR	recently	recently	[Np[Tn[R:t.R:t]
J09:0210c	-	VVNt	sensitized	sensitize	[Vn.Vn]Tn]
J09:0210d	-	NN2	individuals	individual	.Np]P:q]S]
J09:0210e	-	YF	+.	-	.
J09:0210f	-	MC1	One	one	[S[Ms:s.Ms:s]
J09:0210g	-	VVDt	contained	contain	[Vd.Vd]
J09:0210h	-	JJ	complete	complete	[Ns:o.
J09:0210i	-	NN1c	antibody	antibody	.Ns:o]
J09:0220a	-	CC	and	and	[S+.
J09:0220b	-	VHD	had	have	[Vd.Vd]
J09:0220c	-	AT1	a	a	[Ns:o.
J09:0220d	-	NN1n	titer	titre	.
J09:0220e	-	IO	of	of	[Po.
J09:0220f	-	MC1n	1	-	[Ms.
J09:0220g	-	IIx	+:	-	[P.
J09:0220h	-	MCn	+512	-	.P]Ms]Po]Ns:o]
J09:0220i	-	II	in	in	[P:p.
J09:0220j	-	NN1u	saline	saline	.P:p]S+]S]
J09:0220k	-	YF	+.	-	.
J09:0220m	-	AT	The	the	[S[Nj:s.
J09:0220n	-	MDo	second	second	.Nj:s]
J09:0220p	-	VVDt	contained	contain	[Vd.Vd]
J09:0220q	-	JJ	incomplete	incomplete	[Ns:o.
J09:0230a	-	NN1c	antibody	antibody	.Ns:o]
J09:0230b	-	CC	and	and	[S+.
J09:0230c	-	VVDv	showed	show	[Vd.Vd]
J09:0230d	-	NN2	titers	titre	[Np:o.
J09:0230e	-	IO	of	of	[Po.
J09:0230f	-	MC1n	1	-	[Ms.
J09:0230g	-	IIx	+:	-	[P.
J09:0230h	-	MCn	+256	-	.P]Ms]Po]Np:o]
J09:0230i	-	II	in	in	[P:p.
J09:0230j	-	NN1u	albumin	albumin	.P:p]
J09:0230k	-	CC	and	and	[S+.
J09:0230m	-	MC1n	1	-	[Ms:o.
J09:0230n	-	IIx	+:	-	[P.
J09:0230p	-	MCn	+2048	-	.P]Ms:o]
J09:0230q	-	IIb	by	by	[Pb:h.
J09:0240a	-	AT	the	the	[Ns.
J09:0240b	-	JJ	indirect	indirect	.
J09:0240c	-	NP1s	Coombs	Coombs	[Nns.Nns]
J09:0240d	-	NN1c	test	test	.Ns]Pb:h]S+]S+]S]
J09:0240e	-	YF	+.	-	.
J09:0240f	-	AT	The	the	[S[Nj:s111.
J09:0240g	-	MD	third	third	.
J09:0240h	-	YC	+,	-	.
J09:0240i	-	YG	-	-	[Tg[s111.s111]
J09:0240j	-	VVGt	containing	contain	[Vg.Vg]
J09:0240k	-	AT	the	the	[Ns:o.
J09:0240m	-	JJ	mixed	mixed	.
J09:0240n	-	NN1n	type	type	.
J09:0240p	-	IO	of	of	[Po.
J09:0240q	-	JJ	complete	complete	[Np[JJ&.
J09:0250a	-	CC	and	and	[JJ+.
J09:0250b	-	JJ	incomplete	incomplete	.JJ+]JJ&]
J09:0250c	-	NN2	antibodies	antibody	.Np]Po]Ns:o]Tg]
J09:0250d	-	YC	+,	-	.Nj:s111]
J09:0250e	-	VHD	had	have	[Vd.Vd]
J09:0250f	-	NN2	titers	titre	[N:o.
J09:0250g	-	IO	of	of	[Po.
J09:0250h	-	MC1n	1	-	[Ms.
J09:0250i	-	IIx	+:	-	[P.
J09:0250j	-	MCn	+256	-	.P]Ms]Po]
J09:0250k	-	II	in	in	[P.
J09:0250m	-	NN1u	saline	saline	.P]
J09:0250n	-	YC	+,	-	.
J09:0250p	-	MC1n	1	-	[N-[Ms.
J09:0250q	-	IIx	+:	-	[P.
J09:0250r	-	MCn	+512	-	.P]Ms]
J09:0260a	-	II	in	in	[P.
J09:0260b	-	NN1u	albumin	albumin	.P]N-]
J09:0260c	-	CC	and	and	[N+.
J09:0260d	-	MC1n	1	-	[Ms.
J09:0260e	-	IIx	+:	-	[P.
J09:0260f	-	MCn	+1024	-	.P]Ms]
J09:0260g	-	IIb	by	by	[Pb.
J09:0260h	-	AT	the	the	[Ns.
J09:0260i	-	JJ	indirect	indirect	.
J09:0260j	-	NP1s	Coombs	Coombs	[Nns.Nns]
J09:0260k	-	NN1c	test	test	.Ns]Pb]N+]N:o]S]
J09:0260m	-	YF	+.	-	.
J09:0260n	-	II	In	in	[S[P:m.
J09:0260p	-	NN1n	addition	addition	.P:m]
J09:0270a	-	MC1	one	one	[Ns:S.
J09:0270b	-	NN1u	serum	serum	.Ns:S]
J09:0270c	-	VBDZ	was	be	[Vsp.
J09:0270d	-	VVNv	obtained	obtain	.Vsp]
J09:0270e	-	II	from	from	[P:q.
J09:0270f	-	AT1	a	a	[Ns:113.
J09:0270g	-	NN1c	donor	donor	.
J09:0270h	-	YPL	(	-	.
J09:0270i	A	NP1i	+R.	-	[Nns@.
J09:0270j	A	NP1i	E.	-	.Nns@]
J09:0270k	-	YPR	+)	-	.
J09:0270m	-	PNQSr	who	who	[Fr[Nq:S113.Nq:S113]
J09:0270n	-	VHD	had	have	[Vdfp.
J09:0270p	-	VBN	been	be	.
J09:0270q	-	VVNt	sensitized	sensitize	.Vdfp]
J09:0280a	-	MCn	6	-	[Rx:t[Np.
J09:0280b	-	NNT2	years	year	.Np]
J09:0280c	-	RR	previously	previously	.Rx:t]Fr]Ns:113]P:q]S]
J09:0280d	-	YF	+.	-	.
J09:0280e	-	DD1i	This	this	[S[Ns:s.
J09:0280f	-	NN1u	serum	serum	.Ns:s]
J09:0280g	-	VVDt	exhibited	exhibit	[Vd.Vd]
J09:0280h	-	NN2	titers	titre	[N:o.
J09:0280i	-	IO	of	of	[Po.
J09:0280j	-	MC1n	1	-	[Ms.
J09:0280k	-	IIx	+:	-	[P.
J09:0280m	-	MCn	+16	-	.P]Ms]Po]
J09:0280n	-	II	in	in	[P.
J09:0280p	-	NN1u	albumin	albumin	.P]
J09:0290a	-	CC	and	and	[N+.
J09:0290b	-	MC1n	1	-	[Ms.
J09:0290c	-	IIx	+:	-	[P.
J09:0290d	-	MCn	+256	-	.P]Ms]
J09:0290e	-	IIb	by	by	[Pb.
J09:0290f	-	AT	the	the	[Ns.
J09:0290g	-	JJ	indirect	indirect	.
J09:0290h	-	NP1s	Coombs	Coombs	[Nns.Nns]
J09:0290i	-	NN1c	test	test	.Ns]Pb]N+]N:o]S]
J09:0290j	-	YF	+.	-	.
J09:0290k	-	DD2i	These	these	[S[Np:S.
J09:0290m	-	NN1c	antibody	antibody	.
J09:0290n	-	NN2	titers	titre	.Np:S]
J09:0300a	-	VBDR	were	be	[Vwp.
J09:0300b	-	VVNv	determined	determine	.Vwp]
J09:0300c	-	IIb	by	by	[Pb:a.
J09:0300d	-	NN1n	reaction	reaction	[Ns.
J09:0300e	-	IW	with	with	[P.
J09:0300f	-	JJ	homozygous	homozygous	[Np.
J09:0300g	-	FO	<formul>	-	.
J09:0300h	-	JJ	red	red	.
J09:0300i	-	NN2	cells	cell	.Np]P]Ns]Pb:a]S]
J09:0300j	-	YF	+.	-	.O]
J09:0300k	-	YBL	<bminhd>	-	[Oh.
J09:0300m	-	JJ	SEROLOGICAL	serological	[Ns.
J09:0310a	-	NN1n	TECHNIQUE	technique	.Ns]
J09:0310b	-	YF	+.	-	.
J09:0310c	-	YBR	<eminhd>	-	.Oh]
J09:0310d	-	FB	Anti	anti<hyphen>	[O[S[Np:S[J.
J09:0310e	-	YH	+<hyphen>	-	.
J09:0310f	S	ZZ1	+A	-	.
J09:0310g	-	CC	and	and	[J+.
J09:0310h	-	FB	anti	anti<hyphen>	.
J09:0310i	-	YH	+<hyphen>	-	.
J09:0310j	S	ZZ1	+B	-	.J+]J]
J09:0310k	-	NN2	activities	activity	.Np:S]
J09:0310m	-	VBDR	were	be	[Vwp.
J09:0310n	-	VVNv	determined	determine	.Vwp]
J09:0320a	-	II	in	in	[P:p.
J09:0320b	-	NN2	fractions	fraction	[Np.
J09:0320c	-	II	from	from	[P.
J09:0320d	-	AT	the	the	[Np.
J09:0320e	-	NN2	sera	serum	.
J09:0320f	-	IO	of	of	[Po.
J09:0320g	-	NNJ1c	group	group	[Np[Nn.
J09:0320h	S	ZZ1	A	-	.
J09:0320i	-	YC	+,	-	.
J09:0320j	-	NNJ1c	group	group	[Nns-.
J09:0320k	S	ZZ1	B	-	.Nns-]
J09:0320m	-	CCr	or	or	[Nns+.
J09:0320n	-	NNJ1c	group	group	.
J09:0320p	S	ZZ1	O	-	.Nns+]Nn]
J09:0330a	-	NN2	donors	donor	.Np]Po]Np]P]Np]P:p]
J09:0330b	-	IIb	by	by	[Pb:h.
J09:0330c	-	AT	the	the	[Np.
J09:0330d	-	JJ	following	following	.
J09:0330e	-	NN1n	tube	tube	.
J09:0330f	-	NN1n	agglutination	agglutination	.
J09:0330g	-	NN2	methods	method	.Np]Pb:h]S]
J09:0330h	-	YF	+.	-	.
J09:0330i	-	MC1	One	one	[S[Ns:S.
J09:0330j	-	NN1c	drop	drop	.
J09:0330k	-	IO	of	of	[Po.
J09:0330m	-	DD1q	each	each	[Ns.
J09:0340a	-	NN1c	sample	sample	.Ns]Po]Ns:S]
J09:0340b	-	VBDZ	was	be	[Vsp.
J09:0340c	-	VVNv	added	add	.Vsp]
J09:0340d	-	IIt	to	to	[P:q.
J09:0340e	-	MC1	one	one	[Ns.
J09:0340f	-	NN1c	drop	drop	.
J09:0340g	-	IO	of	of	[Po.
J09:0340h	-	AT1	a	a	[Ns.
J09:0340i	-	MCn	2	-	[Nu.
J09:0340j	-	NNUp	+%	-	.Nu]
J09:0340k	-	NN1u	suspension	suspension	.
J09:0340m	-	IO	of	of	[Po.
J09:0340n	-	NNJ1c	group	group	[Np[Nn.
J09:0340p	-	FO	<formul>	-	.
J09:0340q	-	CCr	or	or	[Nns+.
J09:0350a	-	NNJ1c	group	group	.
J09:0350b	S	ZZ1	B	-	.Nns+]Nn]
J09:0350c	-	JJ	red	red	.
J09:0350d	-	NN2	cells	cell	.Np]Po]Ns]Po]Ns]P:q]
J09:0350e	-	II	in	in	[P:p.
J09:0350f	-	AT1	a	a	[Ns.
J09:0350g	-	JJ	small	small	.
J09:0350h	-	FO	<formul>	-	.
J09:0350i	-	NN1c	test	test	.
J09:0350j	-	NN1n	tube	tube	.Ns]P:p]S]
J09:0350k	-	YF	+.	-	.
J09:0350m	-	II	In	in	[S[P:t.
J09:0350n	-	DA2q	several	several	[Np.
J09:0350p	-	NN2	instances	instance	.Np]P:t]
J09:0360a	-	NNJ1c	group	group	[Np:S[Nns.
J09:0360b	S	ZZ1	O	-	.Nns]
J09:0360c	-	NN2	cells	cell	.Np:S]
J09:0360d	-	YG	-	-	[m115.m115]
J09:0360e	-	VBDR	were	be	[Vwp.
J09:0360f	-	RR	also	also	[R:G115.R:G115]
J09:0360g	-	VVNt	used	use	.Vwp]
J09:0360h	-	IIa	as	as	[P:e.
J09:0360i	-	NN2	controls	control	.P:e]S]
J09:0360j	-	YF	+.	-	.
J09:0360k	-	AT	The	the	[S[Np:S.
J09:0360m	-	JJ	red	red	.
J09:0360n	-	NN2	cells	cell	.Np:S]
J09:0360p	-	VBDR	were	be	[Vwp.
J09:0360q	-	VVNt	used	use	.Vwp]
J09:0370a	-	II	within	within	[P:t.
J09:0370b	-	MCn	2	-	[Np.
J09:0370c	-	NNT2	days	day	.
J09:0370d	-	ICSt	after	after	[P.
J09:0370e	-	NN1n	donation	donation	.P]Np]P:t]
J09:0370f	-	CC	and	and	[S+.
J09:0370g	-	VBDR	were	be	[Vwp.
J09:0370h	-	VVNv	washed	wash	.Vwp]
J09:0370i	-	IW	with	with	[P:h.
J09:0370j	-	JJ	large	large	[Np.
J09:0370k	-	NN2	amounts	amount	.
J09:0370m	-	IO	of	of	[Po.
J09:0370n	-	NN1u	saline	saline	.Po]Np]P:h]
J09:0380a	-	ICSt	before	before	[P:t.
J09:0380b	-	NN1n	use	use	.P:t]S+]S]
J09:0380c	-	YF	+.	-	.
J09:0380d	-	AT	The	the	[S[Np:S117.
J09:0380e	-	NN2	mixtures	mixture	.
J09:0380f	-	IO	of	of	[Po.
J09:0380g	-	NN1c	sample	sample	[N.
J09:0380h	-	CC	plus	plus	[Ns+.
J09:0380i	-	NN1c	cell	cell	.
J09:0380j	-	NN1u	suspension	suspension	.Ns+]N]Po]Np:S117]
J09:0380k	-	VBDR	were	be	[Vwp.
J09:0380m	-	VVNv	allowed	allow	.Vwp]
J09:0380n	-	YG	-	-	[Ti:o[s117.s117]
J09:0390a	-	TO	to	to	[Vi.
J09:0390b	-	VV0v	stand	stand	.Vi]
J09:0390c	-	II	at	at	[P:p.
J09:0390d	-	NN1n	room	room	[Ns.
J09:0390e	-	NN1n	temperature	temperature	.Ns]P:p]
J09:0390f	-	IF	for	for	[P:t.
J09:0390g	-	MC1n	1	-	[Nu.
J09:0390h	S	NNU	hr	-	.Nu]P:t]Ti:o]S]
J09:0390i	-	YF	+.	-	.
J09:0390j	-	AT	the	the	[S[Np:s.
J09:0390k	-	NN2	tubes	tube	.Np:s]
J09:0390m	-	YG	-	-	[t118.t118]
J09:0390n	-	VBDR	were	be	[Vwp.
J09:0390p	-	RTn	then	then	[Rsw:G118.Rsw:G118]
J09:0400a	-	VVNt	centrifuged	centrifuge	.Vwp]
J09:0400b	-	II	at	at	[P:h.
J09:0400c	-	MCn	1000	-	[Nu.
J09:0400d	S	NNU	rpm	-	.Nu]P:h]
J09:0400e	-	IF	for	for	[P:t.
J09:0400f	-	MC1n	1	-	[Nu.
J09:0400g	S	NNU	min	-	.Nu]P:t]
J09:0400h	-	CC	and	and	[S+.
J09:0400i	-	VVNt	examined	examine	[Vn.Vn]
J09:0400j	-	RR	macroscopically	macroscopically	[R:h.R:h]
J09:0400k	-	IF	for	for	[P:r.
J09:0410a	-	NN1n	agglutination	agglutination	.P:r]S+]S]
J09:0410b	-	YF	+.	-	.
J09:0410c	-	IF	For	for	[S[P:c.
J09:0410d	-	AT	the	the	[Ns.
J09:0410e	-	NN1u	albumin	albumin	.
J09:0410f	-	NN1n	method	method	.Ns]P:c]
J09:0410g	-	YC	+,	-	.
J09:0410h	-	JJ	equal	equal	[Np:S119.
J09:0410i	-	NN2	volumes	volume	.
J09:0410j	-	IO	of	of	[Po.
J09:0410k	-	MCn	30	-	[N:121[Nu.
J09:0410m	-	NNUp	+%	-	.Nu]
J09:0410n	-	JJ	bovine	bovine	.
J09:0420a	-	NN1u	albumin	albumin	.
J09:0420b	-	YC	+,	-	.
J09:0420c	-	NN1c	sample	sample	[Ns-.Ns-]
J09:0420d	-	CC	and	and	[Np+.
J09:0420e	-	MCn	2	-	[Nu.
J09:0420f	-	NNUp	+%	-	.Nu]
J09:0420g	-	NN2	cells	cell	.
J09:0420h	-	YG	-	-	[Tn[S121.S121]
J09:0420i	-	VVNt	suspended	suspend	[Vn.Vn]
J09:0420j	-	II	in	in	[P:p.
J09:0420k	-	NN1u	saline	saline	.P:p]Tn]Np+]N:121]Po]Np:S119]
J09:0420m	-	VBDR	were	be	[Vwp.
J09:0420n	-	VVNv	allowed	allow	.Vwp]
J09:0420p	-	YG	-	-	[Ti:o[s119.s119]
J09:0420q	-	TO	to	to	[Vi.
J09:0430a	-	VV0v	stand	stand	.Vi]
J09:0430b	-	II	at	at	[P:p.
J09:0430c	-	NN1n	room	room	[Ns.
J09:0430d	-	NN1n	temperature	temperature	.Ns]P:p]
J09:0430e	-	IF	for	for	[P:t.
J09:0430f	-	MC1n	1	-	[Nu.
J09:0430g	S	NNU	hr	-	.Nu]P:t]Ti:o]
J09:0430h	-	CC	and	and	[S+.
J09:0430i	-	RTn	then	then	[Rsw:t.Rsw:t]
J09:0430j	-	VBDR	were	be	[Vwp.
J09:0430k	-	VVNt	centrifuged	centrifuge	.Vwp]
J09:0430m	-	II	at	at	[P:h.
J09:0430n	-	MCn	1000	-	[Nu.
J09:0440a	S	NNU	rpm	-	.Nu]P:h]
J09:0440b	-	IF	for	for	[P:t.
J09:0440c	-	MC1n	1	-	[Nu.
J09:0440d	S	NNU	min	-	.Nu]P:t]S+]S]
J09:0440e	-	YF	+.	-	.
J09:0440f	-	DBa	All	all	[S[Np:S.
J09:0440g	-	NN2	samples	sample	.Np:S]
J09:0440h	-	VBDR	were	be	[Vwp.
J09:0440i	-	VVNt	tested	test	.Vwp]
J09:0440j	-	IIb	by	by	[Pb:a.
J09:0440k	-	DB2	both	both	[Np.
J09:0440m	-	AT	the	the	.
J09:0440n	-	NN1u	saline	saline	[NN1u&.
J09:0440p	-	CC	and	and	[NN1u+.
J09:0450a	-	NN1u	albumin	albumin	.NN1u+]NN1u&]
J09:0450b	-	NN2	methods	method	.Np]Pb:a]S]
J09:0450c	-	YF	+.	-	.
J09:0450d	-	AT	The	the	[S[Np:S.
J09:0450e	-	NN2	activities	activity	.
J09:0450f	-	IO	of	of	[Po.
J09:0450g	-	NN2	fractions	fraction	[Np.
J09:0450h	-	IO	of	of	[Po.
J09:0450i	-	NN2	sera	serum	[Np:123.
J09:0450j	-	YG	-	-	[Tg[s123.s123]
J09:0450k	-	VVGt	containing	contain	[Vg.Vg]
J09:0450m	S	FWs	Rh	-	[Np:o[Nn.Nn]
J09:0460a	-	NN2	antibodies	antibody	.Np:o]Tg]Np:123]Po]Np]Po]Np:S]
J09:0460b	-	VBDR	were	be	[Vwp.
J09:0460c	-	VVNt	tested	test	.Vwp]
J09:0460d	-	IIb	by	by	[Pb:a.
J09:0460e	-	AT	the	the	[Np.
J09:0460f	-	NN1u	saline	saline	[N.
J09:0460g	-	YC	+,	-	.
J09:0460h	-	NN1u	albumin	albumin	[Ns-.Ns-]
J09:0460i	-	CC	and	and	[Nns+.
J09:0460j	-	JJ	indirect	indirect	.
J09:0460k	-	NP1s	Coombs	Coombs	.Nns+]N]
J09:0470a	-	NN2	techniques	technique	.Np]Pb:a]S]
J09:0470b	-	YF	+.	-	.
J09:0470c	-	JJ	Homozygous	homozygous	[S[Np:s[JJ&.
J09:0470d	-	CC	and	and	[JJ+.
J09:0470e	-	JJ	heterozygous	heterozygous	.JJ+]JJ&]
J09:0470f	-	FO	<formul>	-	.
J09:0470g	-	NN2	cells	cell	.
J09:0470h	-	YC	+,	-	.
J09:0470i	-	FO	<formul>	-	[Np-.
J09:0470j	-	CC	and	and	.
J09:0470k	-	JJ	homozygous	homozygous	[JJ&.
J09:0480a	-	CC	and	and	[JJ+.
J09:0480b	-	JJ	heterozygous	heterozygous	.JJ+]JJ&]
J09:0480c	-	FO	<formul>	-	.
J09:0480d	-	NN2	cells	cell	.Np-]Np:s]
J09:0480e	-	VBDR	were	be	[Vwp.
J09:0480f	-	VVNt	used	use	.Vwp]
J09:0480g	-	TO	to	to	[Ti:c[Vi.
J09:0480h	-	VV0t	test	test	.Vi]
J09:0480i	-	DD1q	each	each	[Ns:o.
J09:0480j	-	NN1c	sample	sample	.Ns:o]Ti:c]
J09:0480k	-	YS	+;	-	.
J09:0480m	-	RR	however	however	[S-[R:c.R:c]
J09:0480n	-	YC	+,	-	.
J09:0481a	-	II	in	in	[P:c.
J09:0481b	-	AT	the	the	[Ns.
J09:0490a	-	NN1n	interest	interest	.
J09:0490b	-	IO	of	of	[Po.
J09:0490c	-	NN1u	clarity	clarity	[NN1u&.
J09:0490d	-	CC	and	and	[NN1u+.
J09:0490e	-	NN1u	conciseness	conciseness	.NN1u+]NN1u&]Po]Ns]P:c]
J09:0490f	-	RRx	only	only	[R:m.R:m]
J09:0490g	-	AT	the	the	[Np:S125.
J09:0490h	-	NN2	results	result	.
J09:0490i	-	YG	-	-	[Tn[S125.S125]
J09:0490j	-	VVNv	obtained	obtain	[Vn.Vn]
J09:0490k	-	IW	with	with	[P:h.
J09:0500a	-	JJ	homozygous	homozygous	[Np[N.
J09:0500b	-	FO	<formul>	-	.
J09:0500c	-	CC	and	and	[N+.
J09:0500d	-	JJ	homozygous	homozygous	.
J09:0500e	-	FO	<formul>	-	.N+]N]
J09:0500f	-	NN2	cells	cell	.Np]P:h]Tn]Np:S125]
J09:0500g	-	VMo	will	will	[Vcp.
J09:0500h	-	VB0	be	be	.
J09:0500i	-	VVNt	presented	present	.Vcp]
J09:0500j	-	RLh	here	here	[Rw:p.Rw:p]S-]S]
J09:0500k	-	YF	+.	-	.
J09:0510a	-	AT	The	the	[S[Np:S.
J09:0510b	-	NN1u	saline	saline	[NN1u&.
J09:0510c	-	CC	and	and	[NN1u+.
J09:0510d	-	NN1u	albumin	albumin	.NN1u+]NN1u&]
J09:0510e	-	NN2	tests	test	.Np:S]
J09:0510f	-	VBDR	were	be	[Vwp.
J09:0510g	-	VVNv	performed	perform	.Vwp]
J09:0510h	-	CSA	as	as	[A:h.
J09:0510i	-	VVNt	described	describe	[Vn.Vn]
J09:0510j	-	IF	for	for	[P:r.
J09:0510k	-	AT	the	the	[Np.
J09:0510m	S	FOs	ABO	-	.
J09:0520a	-	NN2	samples	sample	.Np]P:r]A:h]
J09:0520b	-	CS21	except	except	[Fa:m[CS=.
J09:0520c	-	CS22	that	that	.CS=]
J09:0520d	-	AT	the	the	[Ns:S.
J09:0520e	-	NN1n	mixture	mixture	.Ns:S]
J09:0520f	-	VBDZ	was	be	[Vsp.
J09:0520g	-	VVNv	incubated	incubate	.Vsp]
J09:0520h	-	IF	for	for	[P:t.
J09:0520i	-	MC1n	1	-	[Nu.
J09:0520j	S	NNU	hr	-	.Nu]P:t]
J09:0520k	-	II	at	at	[P:p.
J09:0520m	-	MCn	37	-	[Nu.
J09:0520n	S	NNU	+<deg>C	-	.Nu]P:p]
J09:0530a	-	ICSt	before	before	[P:t.
J09:0530b	-	NN1u	centrifugation	centrifugation	.P:t]Fa:m]S]
J09:0530c	-	YF	+.	-	.
J09:0530d	-	AT	The	the	[S[Np:S.
J09:0530e	-	NN1u	saline	saline	.
J09:0530f	-	NN2	tubes	tube	.Np:S]
J09:0530g	-	VBDR	were	be	[Vwp.
J09:0530h	-	VVNv	saved	save	[VVNv&.
J09:0530i	-	CC	and	and	[VVNt+.
J09:0530j	-	VVNt	used	use	.VVNt+]VVNv&]Vwp]
J09:0531a	-	IF	for	for	[P:c.
J09:0540a	-	AT	the	the	[Ns.
J09:0540b	-	JJ	indirect	indirect	.
J09:0540c	-	NP1s	Coombs	Coombs	[Nns.Nns]
J09:0540d	-	NN1c	test	test	.Ns]P:c]
J09:0540e	-	II	in	in	[P:h.
J09:0540f	-	AT	the	the	[Ns.
J09:0540g	-	JJ	following	following	.
J09:0540h	-	NN1c	manner	manner	.Ns]P:h]S]
J09:0540i	-	YF	+.	-	.
J09:0540j	-	AT	The	the	[S[Np:S.
J09:0540k	-	NN2	cells	cell	.Np:S]
J09:0540m	-	VBDR	were	be	[Vwp.
J09:0540n	-	VVNv	washed	wash	.Vwp]
J09:0550a	-	MC	three	three	[Np:t.
J09:0550b	-	NNT2	times	time	.Np:t]
J09:0550c	-	IW	with	with	[P:h.
J09:0550d	-	NN1u	saline	saline	.P:h]
J09:0550e	-	YC	+,	-	.
J09:0550f	-	FB	anti	anti<hyphen>	[S-[Ns:S[J.
J09:0550g	-	YH	+<hyphen>	-	.
J09:0550h	-	JJ	+human	human	.J]
J09:0550i	-	NN1u	serum	serum	.Ns:S]
J09:0550j	-	VBDZ	was	be	[Vsp.
J09:0550k	-	VVNv	added	add	.Vsp]S-]
J09:0550m	-	YC	+,	-	.
J09:0550n	-	AT	the	the	[S-[Np:S.
J09:0550p	-	NN2	cells	cell	.Np:S]
J09:0550q	-	VBDR	were	be	[Vwp.
J09:0560a	-	VVNt	resuspended	resuspend	.Vwp]S-]
J09:0560b	-	YC	+,	-	.
J09:0560c	-	CC	and	and	[S+.
J09:0560d	-	AT	the	the	[Ns:S.
J09:0560e	-	NN1n	mixture	mixture	.Ns:S]
J09:0560f	-	VBDZ	was	be	[Vsp.
J09:0560g	-	VVNt	centrifuged	centrifuge	.Vsp]
J09:0560h	-	II	at	at	[P:h.
J09:0560i	-	MCn	1000	-	[Nu.
J09:0560j	S	NNU	rpm	-	.Nu]P:h]
J09:0560k	-	IF	for	for	[P:t.
J09:0560m	-	MC1n	1	-	[Nu.
J09:0560n	S	NNU	min	-	.Nu]P:t]
J09:0570a	-	CC	and	and	[S+.
J09:0570b	-	VVNt	examined	examine	[Vn.Vn]
J09:0570c	-	IF	for	for	[P:r.
J09:0570d	-	NN1u	agglutination	agglutination	.P:r]S+]S+]S]
J09:0570e	-	YF	+.	-	.
J09:0570f	-	AT	The	the	[S[Np:S127.
J09:0570g	-	FB	anti	anti<hyphen>	[J.
J09:0570h	-	YH	+<hyphen>	-	.
J09:0570i	-	JJ	+human	human	.J]
J09:0570j	-	NN2	sera	serum	.
J09:0570k	-	YG	-	-	[Tn[S127.S127]
J09:0570m	-	VVNt	used	use	[Vn.Vn]Tn]Np:S127]
J09:0570n	-	VBDR	were	be	[Vwp.
J09:0570p	-	VVNv	prepared	prepare	.Vwp]
J09:0580a	-	IIb	by	by	[Pb:q.
J09:0580b	-	VVGt	injecting	inject	[Tg[Vg.Vg]
J09:0580c	-	JJ	whole	whole	[Ns:o.
J09:0580d	-	JJ	human	human	.
J09:0580e	-	NN1u	serum	serum	.Ns:o]
J09:0580f	-	II	into	into	[P:q.
J09:0580g	-	NN2	rabbits	rabbit	.P:q]Tg]Pb:q]S]
J09:0580h	-	YF	+.	-	.
J09:0580i	-	DD2a	Those	those	[S[Np:S129.
J09:0580j	-	NN2	antisera	antiserum	.
J09:0580k	-	YG	-	-	[Tn[S129.S129]
J09:0580m	-	VVNv	shown	show	[Vn.Vn]
J09:0590a	-	IIb	by	by	[Pb:h.
J09:0590b	-	NN1u	immunoelectrophoresis	immunoelectrophoresis	.Pb:h]
J09:0590c	-	YG	-	-	[Ti:o[s129.s129]
J09:0590d	-	TO	to	to	[Vib.
J09:0590e	-	VB0	be	be	.Vib]
J09:0590f	-	IO	of	of	[Po:e.
J09:0590g	-	AT	the	the	[Ns.
J09:0590h	-	YIL	<ldquo>	-	.
J09:0590i	-	JJ	+broad	broad	[Ns.
J09:0590j	-	NN1c	spectrum	spectrum	.Ns]
J09:0590k	-	YIR	+<rdquo>	-	.
J09:0590m	-	NN1n	type	type	.Ns]Po:e]Ti:o]Tn]Np:S129]
J09:0590n	-	VBDR	were	be	[Vwp.
J09:0600a	-	VVNt	selected	select	.Vwp]
J09:0600b	-	IF	for	for	[P:c.
J09:0600c	E	NN1n	used	use	[Ns.
J09:0600d	-	II	in	in	[P.
J09:0600e	-	AT	the	the	[Ns.
J09:0600f	-	JJ	present	present	.
J09:0600g	-	NN1n	study	study	.Ns]P]Ns]P:c]S]
J09:0600h	-	YF	+.	-	.O]
J09:0600i	-	YB	<minbrk>	-	[Oh.Oh]
J09:0600j	-	AT	The	the	[O[S[Np:S.
J09:0600k	-	JJ	red	red	.
J09:0600m	-	NN2	cells	cell	.
J09:0600n	-	IF	for	for	[P.
J09:0600p	-	AT	the	the	[Np.
J09:0610a	S	FWs	Rh	-	[Ns[Nn.Nn]
J09:0610b	-	NN1c	antibody	antibody	.Ns]
J09:0610c	-	NN2	tests	test	.Np]P]Np:S]
J09:0610d	-	VBDR	were	be	[Vwp.
J09:0610e	-	VVNt	used	use	.Vwp]
J09:0610f	-	II	within	within	[P:t.
J09:0610g	-	MCn	3	-	[Np.
J09:0610h	-	NNT2	days	day	.
J09:0610i	-	ICSt	after	after	[P.
J09:0610j	-	VVGv	drawing	draw	[Tg[Vg.Vg]Tg]P]Np]P:t]
J09:0610k	-	II21	except	except	[P:m[II=.
J09:0620a	-	II22	for	for	.II=]
J09:0620b	-	AT	the	the	[Np:131.
J09:0620c	-	FO	<formul>	-	.
J09:0620d	-	NN2	cells	cell	.
J09:0620e	-	YC	+,	-	.
J09:0620f	-	DDQr	which	which	[Fr[Dq:S131.Dq:S131]
J09:0620g	-	VHD	had	have	[Vdfp.
J09:0620h	-	VBN	been	be	.
J09:0620i	-	VVNt	glycerolized	glycerolize	.Vdfp]
J09:0620j	-	CC	and	and	[Fr+.
J09:0620k	-	VVNt	stored	store	[Vn.Vn]
J09:0620m	-	II	at	at	[P:p.
J09:0620n	-	MCn	<minus>20	-	[Nu.
J09:0620p	S	NNU	+<deg>C	-	.Nu]P:p]
J09:0630a	-	IF	for	for	[P:t.
J09:0630b	-	RR	approximately	approximately	[Ns[Ms.
J09:0630c	-	MC1n	1	-	.Ms]
J09:0630d	-	NNT1c	year	year	.Ns]P:t]Fr+]Fr]Np:131]P:m]S]
J09:0630e	-	YF	+.	-	.
J09:0630f	-	DD2i	These	these	[S[Np:S.
J09:0630g	-	NN2	cells	cell	.Np:S]
J09:0630h	-	VBDR	were	be	[Vwp.
J09:0630i	-	VVNv	thawed	thaw	.Vwp]
J09:0630j	-	II	at	at	[P:p.
J09:0630k	-	MCn	37	-	[Nu.
J09:0630m	S	NNU	+<deg>C	-	.Nu]P:p]
J09:0630n	-	IF	for	for	[P:t.
J09:0630p	-	MCn	30	-	[Nu.
J09:0640a	S	NNU	min	-	.Nu]P:t]
J09:0640b	-	CC	and	and	[S+.
J09:0640c	-	VBDR	were	be	[Vwp.
J09:0640d	-	VVNt	deglycerolized	deglycerolize	.Vwp]
J09:0640e	-	IIb	by	by	[Pb:h.
J09:0640f	-	RR	alternately	alternately	.
J09:0640g	-	VVGt	centrifuging	centrifuge	[Tg[Vg.Vg]
J09:0640h	-	CC	and	and	[Tg+.
J09:0640i	-	VVGv	mixing	mix	[Vg.Vg]
J09:0650a	-	IW	with	with	[P:w.
J09:0650b	-	VVGv	descending	descend	[Np[Tg[Vg.Vg]Tg]
J09:0650c	-	NN2	concentrations	concentration	.
J09:0650d	-	IO	of	of	[Po.
J09:0650e	-	NN1u	glycerol	glycerol	[Np.
J09:0650f	-	NN2	solutions	solution	.
J09:0650g	-	YPL	(	-	.
J09:0650h	-	MCn	+20	-	[Nu@[MCn&.
J09:0650i	-	YC	+,	-	.
J09:0650j	-	MCn	18	-	[MCn-.MCn-]
J09:0650k	-	YC	+,	-	.
J09:0650m	-	MCn	10	-	[MCn-.MCn-]
J09:0650n	-	YC	+,	-	.
J09:0650p	-	MCn	8	-	[MCn-.MCn-]
J09:0650q	-	YC	+,	-	.
J09:0650r	-	MCn	4	-	[MCn-.MCn-]
J09:0660a	-	CC	and	and	[MCn+.
J09:0660b	-	MCn	2	-	.MCn+]MCn&]
J09:0660c	-	NNUp	+%	-	.Nu@]
J09:0660d	-	YPR	+)	-	.Np]Po]Np]P:w]Tg+]Tg]Pb:h]S+]S]
J09:0660e	-	YF	+.	-	.
J09:0660f	-	AT	The	the	[S[Np:S.
J09:0660g	-	NN2	cells	cell	.Np:S]
J09:0660h	-	YG	-	-	[t133.t133]
J09:0660i	-	VBDR	were	be	[Vwp.
J09:0660j	-	RTn	then	then	[Rsw:G133.Rsw:G133]
J09:0660k	-	VVNv	washed	wash	.Vwp]
J09:0660m	-	MC	three	three	[Np:t.
J09:0660n	-	NNT2	times	time	.Np:t]
J09:0660p	-	IW	with	with	[P:h.
J09:0660q	-	NN1u	saline	saline	.P:h]
J09:0660r	-	CC	and	and	[S+.
J09:0660s	-	VVNt	resuspended	resuspend	[Vn.Vn]
J09:0670a	-	IIt	to	to	[P:q.
J09:0670b	-	MCn	2	-	[Nu.
J09:0670c	-	NNUp	+%	-	.Nu]P:q]
J09:0670d	-	II	in	in	[P:p.
J09:0670e	-	NN1u	saline	saline	.P:p]S+]S]
J09:0670f	-	YF	+.	-	.O]
J09:0670g	-	YBL	<bminhd>	-	[Oh.
J09:0670h	-	NN1u	CHROMATOGRAPHY	chromatography	.
J09:0670i	-	YF	+.	-	.
J09:0670j	-	YBR	<eminhd>	-	.Oh]
J09:0670k	-	NN1n	Blood	blood	[O[S[Np:S135.
J09:0670m	-	NN2	samples	sample	.Np:S135]
J09:0670n	-	VBDR	were	be	[Vwp.
J09:0680a	-	VVNv	allowed	allow	.Vwp]
J09:0680b	-	YG	-	-	[Ti:o[s135.s135]
J09:0680c	-	TO	to	to	[Vi.
J09:0680d	-	VV0v	clot	clot	.Vi]
J09:0680e	-	II	at	at	[P:p.
J09:0680f	-	NN1n	room	room	[Ns.
J09:0680g	-	NN1n	temperature	temperature	.Ns]P:p]
J09:0680h	-	IF	for	for	[P:t.
J09:0680i	-	MCn	3	-	[Nu.
J09:0680j	S	NNU	hr	-	.Nu]P:t]Ti:o]
J09:0680k	-	YC	+,	-	.
J09:0680m	-	VVNt	centrifuged	centrifuge	[S-[Vn.Vn]S-]
J09:0680n	-	CC	and	and	[S+.
J09:0680p	-	AT	the	the	[Ns:S.
J09:0681a	-	NN1u	serum	serum	.Ns:S]
J09:0690a	-	VBDZ	was	be	[Vsp.
J09:0690b	-	VVNv	removed	remove	.Vsp]S+]S]
J09:0690c	-	YF	+.	-	.
J09:0690d	-	AT	The	the	[S[Ns:S.
J09:0690e	-	NN1u	serum	serum	.Ns:S]
J09:0690f	-	VBDZ	was	be	[Vsp.
J09:0690g	-	VVNv	measured	measure	.Vsp]
J09:0690h	-	RR	volumetrically	volumetrically	[R:h.R:h]
J09:0690i	-	CC	and	and	[S+.
J09:0690j	-	RR	subsequently	subsequently	[R:t.R:t]
J09:0700a	-	VVNt	dialyzed	dialyse	[Vn.Vn]
J09:0700b	-	II	in	in	[P:p.
J09:0700c	-	AT	the	the	[Ns.
J09:0700d	-	NN1n	cold	cold	.Ns]P:p]
J09:0700e	-	IF	for	for	[P:t.
J09:0700f	-	RR21	at	at	[RR=.
J09:0700g	-	RR22	least	least	.RR=]
J09:0700h	-	MCn	24	-	[Nu.
J09:0700i	S	NNU	hr	-	.Nu]P:t]
J09:0700j	-	II	against	against	[P:h.
J09:0700k	-	MC	three	three	[Np:136[M.
J09:0700m	-	IIt	to	to	[P.
J09:0700n	-	MC	four	four	.P]M]
J09:0700p	-	NN2	changes	change	.
J09:0700q	-	YC	+,	-	.
J09:0700r	-	YG	-	-	[L[s136.s136]
J09:0710a	-	RR	approximately	approximately	[Nu:e.
J09:0710b	-	MCn	750	-	.
J09:0710c	S	NNU	ml	-	.Nu:e]
J09:0710d	-	RAq	each	each	[R:h.R:h]L]
J09:0710e	-	YC	+,	-	.
J09:0710f	-	IO	of	of	[Po.
J09:0710g	-	YIL	<ldquo>	-	.
J09:0710h	-	VVGv	+starting	start	[Ns[Tg[Vg.Vg]Tg]
J09:0710i	-	NN1c	buffer	buffer	.Ns]
J09:0710j	-	YIR	+<rdquo>	-	.Po]Np:136]P:h]S+]S]
J09:0710k	-	YF	+.	-	.
J09:0710m	-	DD1i	This	this	[S[Ns:s.
J09:0720a	-	NN1c	buffer	buffer	.
J09:0720b	-	YC	+,	-	.
J09:0720c	S	NNUb	pH	-	[Nu@.
J09:0720d	-	MCd	8.6	-	.Nu@]
J09:0720e	-	YC	+,	-	.Ns:s]
J09:0720f	-	VBDZ	was	be	[Vsb.Vsb]
J09:0720g	-	MCd	0.005	-	[Nu:e.
J09:0720h	S	NNU	M	-	.
J09:0720i	-	II	in	in	[P.
J09:0720j	-	FO	<formul>	-	.P]
J09:0720k	-	CC	and	and	[Nu+.
J09:0720m	-	MCd	0.039	-	.
J09:0720n	S	NNU	M	-	.
J09:0720p	-	II	in	in	[P.
J09:0725a	-	NN1u	tris(hydroxymethyl)<hyphen>aminomethane	tris(hydroxymethyl)<hyphen>aminomethane	[Ns.
J09:0730a	-	YPL	(	-	.
J09:0730b	-	NN1u	+Tris	Tris	.
J09:0730c	-	YPR	+)	-	.Ns]P]Nu+]Nu:e]S]
J09:0730d	-	YF	+.	-	.
J09:0730e	-	ICSt	After	after	[S[P:t.
J09:0730f	-	NN1u	dialysis	dialysis	.P:t]
J09:0730g	-	AT	the	the	[Ns:S.
J09:0730h	-	NN1c	sample	sample	.Ns:S]
J09:0730i	-	VBDZ	was	be	[Vsp.
J09:0730j	-	VVNt	centrifuged	centrifuge	.Vsp]
J09:0740a	-	CC	and	and	[S+.
J09:0740b	-	AT	the	the	[Ns:s.
J09:0740c	-	NN1n	supernatant	supernatant	.Ns:s]
J09:0740d	-	VVNt	placed	place	[Vn.Vn]
J09:0740e	-	II	on	on	[P:q.
J09:0740f	-	AT1	a	a	[Ns.
J09:0740g	-	FO	<formul>	-	[Nu.
J09:0740h	S	NNU	cm	-	.Nu]
J09:0740i	-	NN1c	column	column	.
J09:0740j	-	IO	of	of	[Po.
J09:0740k	S	FOc	DEAE	-	[Ns:137.
J09:0740m	-	YH	+<hyphen>	-	.
J09:0740n	-	NN1u	+cellulose	cellulose	.
J09:0740p	-	YG	-	-	[Tn[S137.S137]
J09:0750a	-	VVNt	equilibrated	equilibrate	[Vn.Vn]
J09:0750b	-	IW	with	with	[P:h.
J09:0750c	-	VVGv	starting	start	[Ns[Tg[Vg.Vg]Tg]
J09:0750d	-	NN1c	buffer	buffer	.Ns]P:h]Tn]Ns:137]Po]Ns]P:q]S+]S]
J09:0750e	-	YF	+.	-	.
J09:0750f	-	AT	The	the	[S[Ns:S139.
J09:0750g	S	FOc	DEAE	-	[Ns.
J09:0750h	-	YH	+<hyphen>	-	.
J09:0750i	-	NN1u	+cellulose	cellulose	.Ns]
J09:0750j	-	YC	+,	-	.
J09:0750k	-	YG	-	-	[Tg[s139.s139]
J09:0760a	-	VVGt	containing	contain	[Vg.Vg]
J09:0760b	-	MCd	0.78	-	[Nu:o.
J09:0760c	S	NNU	mEq	-	.
J09:0760d	-	IO	of	of	[Po.
J09:0760e	S	FOc	N	-	[Nu.
J09:0760f	-	IIp	+<sol>	-	[P.
J09:0760g	S	NNU	+g	-	.P]Nu]Po]Nu:o]Tg]
J09:0760h	-	YC	+,	-	.Ns:S139]
J09:0760i	-	VBDZ	was	be	[Vsp.
J09:0760j	-	VVNv	prepared	prepare	.Vsp]
J09:0760k	-	II	in	in	[P:p.
J09:0760m	-	APPGi2	our	our	[Ns.
J09:0760n	-	NN1c	laboratory	laboratory	.Ns]P:p]
J09:0770a	-	IIb	by	by	[Pb:h.
J09:0770b	-	AT	the	the	[Ns.
J09:0770c	-	NN1n	method	method	.
J09:0770d	-	IO	of	of	[Po.
J09:0770e	-	NP1s	Peterson	Peterson	[Nn[NP1s&.
J09:0770f	-	CC	and	and	[NP1s+.
J09:0770g	-	NP1s	Sober	Sober	.NP1s+]NP1s&]
J09:0770h	-	YPL	(	-	.
J09:0770i	-	MCn	+7	-	[Iu.Iu]
J09:0770j	-	YPR	+)	-	.Nn]Po]Ns]Pb:h]
J09:0770k	-	II	from	from	[P:h.
J09:0770m	-	JJ	powdered	powdered	[Ns.
J09:0770n	-	NN1u	cellulose	cellulose	.
J09:0770p	-	YC	+,	-	.
J09:0770q	-	MCn	100	-	[Ns@.
J09:0770r	-	YH	+<hyphen>	-	[P.
J09:0770s	-	MCn	+230	-	.P]
J09:0780a	-	NN1c	mesh	mesh	.Ns@]Ns]P:h]S]
J09:0780b	-	YF	+.	-	.
J09:0780c	-	AT	The	the	[S[Ns:S141.
J09:0780d	-	JJ	small	small	.
J09:0780e	-	NN1c	amount	amount	.
J09:0780f	-	IO	of	of	[Po.
J09:0780g	-	JJ	insoluble	insoluble	[Ns.
J09:0780h	-	NN1n	material	material	.Ns]Po]
J09:0780i	-	DDQr	which	which	[Fr[Dq:s141.Dq:s141]
J09:0780j	-	VVDt	precipitated	precipitate	[Vd.Vd]
J09:0790a	-	II	during	during	[P:t.
J09:0790b	-	NN1u	dialysis	dialysis	.P:t]Fr]Ns:S141]
J09:0790c	-	VBDZ	was	be	[Vsp.
J09:0790d	-	VVNt	suspended	suspend	.Vsp]
J09:0790e	-	II	in	in	[P:p.
J09:0790f	-	RR	approximately	approximately	[Nu[M.
J09:0790g	-	MCn	5	-	.M]
J09:0790h	S	NNU	ml	-	.
J09:0790i	-	IO	of	of	[Po.
J09:0790j	-	VVGv	starting	start	[Ns[Tg[Vg.Vg]Tg]
J09:0790k	-	NN1c	buffer	buffer	.Ns]Po]Nu]P:p]
J09:0790m	-	YC	+,	-	.
J09:0800a	-	VVNt	centrifuged	centrifuge	[S-[Vn.Vn]S-]
J09:0800b	-	YC	+,	-	.
J09:0800c	-	VVNt	resuspended	resuspend	[S-[Vn.Vn]
J09:0800d	-	II	in	in	[P:p.
J09:0800e	-	MCd	2.5	-	[Nu.
J09:0800f	S	NNU	ml	-	.
J09:0800g	-	IO	of	of	[Po.
J09:0800h	-	JJ	isotonic	isotonic	[Ns.
J09:0800i	-	NN1u	saline	saline	.Ns]Po]Nu]P:p]S-]
J09:0800j	-	CC	and	and	[S+.
J09:0800k	-	VVNt	tested	test	[Vn.Vn]
J09:0810a	-	IF	for	for	[P:r.
J09:0810b	-	NN1c	antibody	antibody	[Ns.
J09:0810c	-	NN1n	activity	activity	.Ns]P:r]S+]S]
J09:0810d	-	YF	+.	-	.O]
J09:0810e	-	YB	<minbrk>	-	[Oh.Oh]
J09:0810f	-	AT	The	the	[O[S[Ns:s.
J09:0810g	-	NN1u	chromatography	chromatography	.Ns:s]
J09:0810h	-	VBDZ	was	be	[Vsp.
J09:0810i	-	VDN	done	do	.Vsp]
J09:0810j	-	II	at	at	[P:p.
J09:0810k	-	MCn	6	-	[Nu.
J09:0810m	S	NNU	+<deg>C	-	.Nu]P:p]
J09:0820a	-	VVGt	using	use	[Tg:h[Vg.Vg]
J09:0820b	-	NN1c	gradient	gradient	[Ns:o.
J09:0820c	-	NN1n	elution	elution	.Ns:o]
J09:0820d	-	YC	+,	-	.
J09:0820e	-	RR	essentially	essentially	[R:m.R:m]
J09:0820f	-	II21	according	accord	[P:h[II=.
J09:0820g	-	II22	to	to	.II=]
J09:0820h	-	NP1s	Sober	Sober	[Nn[NP1s&.
J09:0820i	-	CC	and	and	[NP1s+.
J09:0820j	-	NP1s	Peterson	Peterson	.NP1s+]NP1s&]Nn]P:h]Tg:h]S]
J09:0820k	-	YF	+.	-	.
J09:0830a	-	AT	The	the	[S[Ns:S143.
J09:0830b	-	JJ	deep	deep	.
J09:0830c	-	JJ	concave	concave	.
J09:0830d	-	NN1c	gradient	gradient	.
J09:0830e	-	YG	-	-	[Tn[S143.S143]
J09:0830f	-	VVNt	employed	employ	[Vn.Vn]Tn]
J09:0830g	-	YPL	(	-	.
J09:0830h	A	NNm	+fig.	-	[Iu[Nns.
J09:0830i	-	MCn	2	-	.Nns]Iu]
J09:0830j	-	YPR	+)	-	.Ns:S143]
J09:0830k	-	VBDZ	was	be	[Vsp.
J09:0830m	-	VVNv	obtained	obtain	.Vsp]
J09:0830n	-	IW	with	with	[P:h.
J09:0840a	-	AT1	a	a	[Ns.
J09:0840b	-	MC	nine	nine	[J.
J09:0840c	-	YH	+<hyphen>	-	.
J09:0840d	-	JJh	+chambered	chamber	.J]
J09:0840e	-	NN1c	gradient	gradient	.
J09:0840f	-	NN1n	elution	elution	.
J09:0840g	-	NN1c	device	device	.
J09:0840h	-	YPL	(	-	.
J09:0840i	-	YIL	+<ldquo>	-	[Nns@.
J09:0840j	-	NP1x	+Varigrad	Varigrad	.
J09:0840k	-	YIR	+<rdquo>	-	.
J09:0840m	-	YC	+,	-	.
J09:0840n	-	NN1n	reference	reference	[Iu[Nns.
J09:0850a	-	MCb	(8)	-	.Nns]Iu]Nns@]
J09:0850b	-	YPR	+)	-	.Ns]P:h]
J09:0850c	-	CC	and	and	[S+.
J09:0850d	-	VHZ	has	have	[Vzfp.
J09:0850e	-	VBN	been	be	.
J09:0850f	-	VVNt	described	describe	.Vzfp]
J09:0850g	-	RLe	elsewhere	elsewhere	[Rw:p.Rw:p]S+]S]
J09:0850h	-	YF	+.	-	.
J09:0850i	-	AT	The	the	[S[Nj:S.
J09:0850j	-	JBo	other	other	.
J09:0850k	-	YC	+,	-	.
J09:0850m	-	AT1	a	a	[Ns@.
J09:0850n	-	JJ	shallow	shallow	.
J09:0850p	-	JJ	concave	concave	.
J09:0860a	-	NN1c	gradient	gradient	.
J09:0860b	-	YPL	(	-	.
J09:0860c	A	NNm	+Fig.	-	[Iu[Nns.
J09:0860d	-	MC1n	1	-	.Nns]Iu]
J09:0860e	-	YPR	+)	-	.Ns@]
J09:0860f	-	YC	+,	-	.Nj:S]
J09:0860g	-	VBDZ	was	be	[Vsp.
J09:0860h	-	VVNv	produced	produce	.Vsp]
J09:0860i	-	IW	with	with	[P:h.
J09:0860j	-	AT1	a	a	[Ns.
J09:0860k	-	JB	so<hyphen>called	so<hyphen>called	.
J09:0860m	-	YIL	<ldquo>	-	.
J09:0860n	-	NN1c	+cone	cone	[N.
J09:0860p	-	YH	+<hyphen>	-	.
J09:0860q	-	NN1c	+sphere	sphere	.N]
J09:0860r	-	YIR	+<rdquo>	-	.
J09:0870a	-	NN1n	apparatus	apparatus	.Ns]P:h]
J09:0870b	-	YC	+,	-	.
J09:0870c	-	AT	the	the	[Tg:b[Ns:s.
J09:0870d	-	YIL	<ldquo>	-	.
J09:0870e	-	NN1c	+cone	cone	.
J09:0870f	-	YIR	+<rdquo>	-	.Ns:s]
J09:0870g	-	VBG	being	be	[Vgb.Vgb]
J09:0870h	-	AT1	a	a	[Ns:e.
J09:0870i	-	MCn	2	-	[Nu.
J09:0870j	-	YH	+<hyphen>	-	.
J09:0870k	-	NNU1c	+liter	litre	.Nu]
J09:0870m	-	NP1s	Erlenmeyer	Erlenmeyer	[Nns.Nns]
J09:0870n	-	NN1c	flask	flask	.Ns:e]
J09:0880a	-	CC	and	and	[Tg+.
J09:0880b	-	AT	the	the	[Ns:s.
J09:0880c	-	YIL	<ldquo>	-	.
J09:0880d	-	NN1c	+sphere	sphere	.Ns:s]
J09:0880e	-	YC	+,	-	.
J09:0880f	-	YIR	+<rdquo>	-	.
J09:0880g	-	AT1	a	a	[Ns:e.
J09:0880h	-	MCn	2	-	[Nu.
J09:0880i	-	YH	+<hyphen>	-	.
J09:0880j	-	NNU1c	+liter	litre	.Nu]
J09:0880k	-	JJ	round	round	[Ns.
J09:0880m	-	YH	+<hyphen>	-	.
J09:0880n	-	NN1c	+bottom	bottom	.Ns]
J09:0880p	-	NN1c	flask	flask	.Ns:e]Tg+]Tg:b]S]
J09:0880q	-	YF	+.	-	.
J09:0880r	-	DD1q	Each	each	[S[Ds:s.Ds:s]
J09:0880s	-	RR	initially	initially	[R:t.R:t]
J09:0880t	-	VVDt	contained	contain	[Vd.Vd]
J09:0890a	-	MCy	1700	-	[Nu:o.
J09:0890b	S	NNU	ml	-	.
J09:0890c	-	IO	of	of	[Po.
J09:0890d	-	NN1c	buffer	buffer	.Po]Nu:o]
J09:0890e	-	YS	+;	-	.
J09:0890f	-	II	in	in	[S@[P:p.
J09:0890g	-	AT	the	the	[Ns.
J09:0890h	-	NN1c	sphere	sphere	.Ns]P:p]
J09:0890i	-	VBDZ	was	be	[Vsb.Vsb]
J09:0890j	-	VVGv	starting	start	[Ns:s[Tg[Vg.Vg]Tg]
J09:0890k	-	NN1c	buffer	buffer	.Ns:s]
J09:0890m	-	CC	and	and	[S+.
J09:0900a	-	II	in	in	[P:p.
J09:0900b	-	AT	the	the	[Ns.
J09:0900c	-	NN1c	cone	cone	.Ns]P:p]
J09:0900d	-	VBDZ	was	be	[Vsb.Vsb]
J09:0900e	-	JJ	final	final	[Ns:s.
J09:0900f	-	NN1c	buffer	buffer	.Ns:s]
J09:0900g	-	YC	+,	-	.
J09:0900h	-	MCd	0.50	-	[L:b[Nu:s.
J09:0900i	S	NNU	M	-	.Nu:s]
J09:0900j	-	II	in	in	[P:r.
J09:0900k	-	DB2	both	both	[N.
J09:0900m	-	FOc	<formul>	-	[FOc&.
J09:0900n	-	CC	and	and	[NN1u+.
J09:0900p	-	NN1u	Tris	Tris	.NN1u+]FOc&]N]P:r]
J09:0900q	-	YC	+,	-	.
J09:0900r	S	NNUb	pH	-	[Nu@.
J09:0901a	-	MCd	4.1	-	.Nu@]L:b]S+]S@]S]
J09:0901b	-	YF	+.	-	.O]
J09:0910a	-	YB	<minbrk>	-	[Oh.Oh]
J09:0910b	-	AT1	A	a	[O[S[Ns:S.
J09:0910c	-	NN1c	flow	flow	.
J09:0910d	-	NN1c	rate	rate	.
J09:0910e	-	IO	of	of	[Po.
J09:0910f	-	MCn	72	-	[N.
J09:0910g	-	FO	<formul>	-	.N]Po]Ns:S]
J09:0910h	-	VBDZ	was	be	[Vsp.
J09:0910i	-	VVNt	used	use	.Vsp]
J09:0910j	-	CC	and	and	[S+.
J09:0910k	-	MCn	12	-	[Np:S[Nu.
J09:0910m	S	NNU	ml	-	.Nu]
J09:0910n	-	NN2	fractions	fraction	.Np:S]
J09:0910p	-	VBDR	were	be	[Vwp.
J09:0910q	-	VVNv	collected	collect	.Vwp]S+]S]
J09:0910r	-	YF	+.	-	.
J09:0920a	-	RR	Approximately	approximately	[S[Np:S[M.
J09:0920b	-	MCn	165	-	.M]
J09:0920c	-	NN2	fractions	fraction	.Np:S]
J09:0920d	-	VBDR	were	be	[Vwp.
J09:0920e	-	VVNv	obtained	obtain	.Vwp]
J09:0920f	-	II	from	from	[P:q.
J09:0920g	-	DD1q	each	each	[Ns.
J09:0920h	-	NN1c	column	column	.Ns]P:q]S]
J09:0920i	-	YF	+.	-	.
J09:0930a	-	DD2i	These	these	[S[Dp:S.Dp:S]
J09:0930b	-	VBDR	were	be	[Vwp.
J09:0930c	-	VVNv	read	read	.Vwp]
J09:0930d	-	II	at	at	[P:p.
J09:0930e	-	MCn	280	-	[Nu.
J09:0930f	S	NNU	m<mgr>	-	.Nu]P:p]
J09:0930g	-	II	in	in	[P:p.
J09:0930h	-	AT1	a	a	[Ns.
J09:0930i	-	NP1s	Beckman	Beckman	[Nns.Nns]
J09:0930j	-	NN1c	model	model	[Nns.
J09:0930k	S	NP1z	DU	-	.Nns]
J09:0930m	-	NN1c	spectrophotometer	spectrophotometer	.Ns]P:p]
J09:0940a	-	CC	and	and	[S+.
J09:0940b	-	VVNt	tested	test	[Vn.Vn]
J09:0940c	-	IF	for	for	[P:r.
J09:0940d	-	NN1c	antibody	antibody	[Ns.
J09:0940e	-	NN1n	activity	activity	.Ns]P:r]
J09:0940f	-	CSA	as	as	[A:h.
J09:0940g	-	VVNt	described	describe	[Vn.Vn]
J09:0940h	-	RL	above	above	[R:p.R:p]A:h]S+]S]
J09:0940i	-	YF	+.	-	.O]
J09:0940j	-	YBL	<bminhd>	-	[Oh.
J09:0940k	-	NN1n	PAPER	paper	[Ns.
J09:0950a	-	NN1u	ELECTROPHORESIS	electrophoresis	.Ns]
J09:0950b	-	YF	+.	-	.
J09:0950c	-	YBR	<eminhd>	-	.Oh]
J09:0950d	-	IF	For	for	[O[S[P:c.
J09:0950e	-	NN1n	protein	protein	[Ns.
J09:0950f	-	NN1u	identification	identification	.Ns]P:c]
J09:0950g	-	YC	+,	-	.
J09:0950h	-	NN2	fractions	fraction	[Np:S.
J09:0950i	-	II	from	from	[P.
J09:0950j	-	AT	the	the	[Ns.
J09:0960a	-	NN1c	column	column	.Ns]P]Np:S]
J09:0960b	-	VBDR	were	be	[Vwp.
J09:0960c	-	VVNv	concentrated	concentrate	.Vwp]
J09:0960d	-	IIb	by	by	[Pb:h.
J09:0960e	-	NN1u	pervaporation	pervaporation	[Ns.
J09:0960f	-	II	against	against	[P.
J09:0960g	-	AT1	a	a	[Ns.
J09:0960h	-	NN1c	stream	stream	.
J09:0960i	-	IO	of	of	[Po.
J09:0960j	-	NN1n	air	air	.Po]Ns]P]
J09:0960k	-	II	at	at	[P.
J09:0960m	-	MCn	5	-	[Nu.
J09:0960n	S	NNU	+<deg>C	-	.Nu]P]Ns]
J09:0970a	-	CCr	or	or	[Pb+.
J09:0970b	-	IIb	by	by	.
J09:0970c	-	JJ	negative	negative	[Ns[Ns.
J09:0970d	-	NN1n	pressure	pressure	.Ns]
J09:0970e	-	NN1u	dialysis	dialysis	.
J09:0970f	-	II	in	in	[P.
J09:0970g	-	AT1	an	an	[Ns:145.
J09:0970h	-	NN1n	apparatus	apparatus	.
J09:0970i	-	DDQr	which	which	[Fr[Dq:s145.Dq:s145]
J09:0970j	-	VVDv	permitted	permit	[Vd.Vd]
J09:0980a	-	JJ	simultaneous	simultaneous	[N:o.
J09:0980b	-	NN1n	concentration	concentration	.
J09:0980c	-	IO	of	of	[Po.
J09:0980d	-	AT	the	the	[Ns.
J09:0980e	-	NN1n	protein	protein	.Ns]Po]
J09:0980f	-	CC	and	and	[Ns+.
J09:0980g	-	NN1u	dialysis	dialysis	.
J09:0980h	-	II	against	against	[P.
J09:0980i	-	JJ	isotonic	isotonic	[Ns.
J09:0990a	-	NN1u	saline	saline	.Ns]P]Ns+]N:o]Fr]Ns:145]P]Ns]Pb+]Pb:h]S]
J09:0990b	-	YF	+.	-	.
J09:0990c	-	II	During	during	[S[P:t.
J09:0990d	-	AT	the	the	[Ns.
J09:0990e	-	DAr	latter	latter	.
J09:0990f	-	NN1n	procedure	procedure	.Ns]P:t]
J09:0990g	-	AT	the	the	[Ns:S.
J09:0990h	-	NN1n	temperature	temperature	.Ns:S]
J09:0990i	-	VBDZ	was	be	[Vsp.
J09:0990j	-	VVNt	maintained	maintain	.Vsp]
J09:1000a	-	II	at	at	[P:p.
J09:1000b	-	MCn	2	-	[Nu.
J09:1000c	S	NNU	+<deg>C	-	.Nu]P:p]
J09:1000d	-	IIb	by	by	[Pb:h.
J09:1000e	-	VVGt	surrounding	surround	[Tg[Vg.Vg]
J09:1000f	-	AT	the	the	[Ns:o.
J09:1000g	-	NN1n	apparatus	apparatus	.Ns:o]
J09:1000h	-	IW	with	with	[P:h.
J09:1000i	-	NN1n	ice	ice	.P:h]Tg]Pb:h]S]
J09:1000j	-	YF	+.	-	.
J09:1000k	-	CS	Because	because	[S[Fa:c.
J09:1000m	-	JJ	negative	negative	[Ns:s[Ns.
J09:1001a	-	NN1n	pressure	pressure	.Ns]
J09:1010a	-	NN1u	dialysis	dialysis	.Ns:s]
J09:1010b	-	VVDv	gave	give	[Vd.Vd]
J09:1010c	-	JJR	better	good	[Ns:o.
J09:1010d	-	NN1n	recovery	recovery	.
J09:1010e	-	IO	of	of	[Po.
J09:1010f	-	NN2	proteins	protein	.Po]Ns:o]
J09:1010g	-	YC	+,	-	.
J09:1010h	-	VVDv	permitted	permit	[Fa-[Vd.Vd]
J09:1010i	-	NN1u	detection	detection	[Ns:o.
J09:1020a	-	IO	of	of	[Po.
J09:1020b	-	NN2	proteins	protein	[Np:147.
J09:1020c	-	YG	-	-	[Tn[S147.S147]
J09:1020d	-	VVNv	concentrated	concentrate	[Vn.Vn]
J09:1020e	-	II	from	from	[P:h.
J09:1020f	-	RG	very	very	[Np[J.
J09:1020g	-	JJ	dilute	dilute	.J]
J09:1020h	-	NN2	solutions	solution	.Np]P:h]Tn]Np:147]Po]Ns:o]Fa-]
J09:1020i	-	CC	and	and	[Fa+.
J09:1020j	-	VBDZ	was	be	[Vsb.Vsb]
J09:1020k	-	AT1	a	a	[Ns:e.
J09:1020m	-	JJR	gentler	gentle	.
J09:1030a	-	NN1n	procedure	procedure	.Ns:e]Fa+]Fa:c]
J09:1030b	-	YC	+,	-	.
J09:1030c	-	PPH1	it	it	[Ni:S.Ni:S]
J09:1030d	-	VBDZ	was	be	[Vsp.
J09:1030e	-	VVNt	used	use	.Vsp]
J09:1030f	-	II	in	in	[P:p.
J09:1030g	-	DBa	all	all	[Np[D.
J09:1030h	-	ICSx	but	but	[P.
J09:1030i	-	AT	the	the	[Nj.
J09:1030j	-	JJT	earliest	early	.Nj]P]D]
J09:1030k	-	NN2	experiments	experiment	.Np]P:p]S]
J09:1030m	-	YF	+.	-	.O]
J09:1030n	-	YB	<minbrk>	-	[Oh.Oh]
J09:1030p	-	NN1n	Paper	paper	[O[S[Ns:S.
J09:1040a	-	NN1u	electrophoresis	electrophoresis	.Ns:S]
J09:1040b	-	VBDZ	was	be	[Vsp.
J09:1040c	-	VVNv	carried	carry	.Vsp]
J09:1040d	-	RP	out	out	[R:n.R:n]
J09:1040e	-	II	on	on	[P:u.
J09:1040f	-	AT	the	the	[Np.
J09:1040g	-	JJ	concentrated	concentrated	.
J09:1040h	-	NN2	samples	sample	.Np]P:u]
J09:1040i	-	II	in	in	[P:p.
J09:1040j	-	AT1	a	a	[Ns.
J09:1040k	-	NP1j	Spinco	Spinco	[Nns.Nns]
J09:1050a	-	NN1c	model	model	[Nns.
J09:1050b	S	FOs	R	-	.Nns]
J09:1050c	-	NN1c	cell	cell	.Ns]P:p]
J09:1050d	-	VVGt	using	use	[Tg:h[Vg.Vg]
J09:1050e	-	NN1u	barbital	barbital	[Ns:o.
J09:1050f	-	NN1c	buffer	buffer	.
J09:1050g	-	YC	+,	-	.
J09:1050h	S	NNUb	pH	-	[Nu@.
J09:1050i	-	MCd	8.6	-	.Nu@]
J09:1050j	-	YC	+,	-	.
J09:1050k	-	JJ	ionic	ionic	[L[Ns:s.
J09:1050m	-	NN1n	strength	strength	.Ns:s]
J09:1060a	-	MCd	0.075	-	[M:e.M:e]L]
J09:1060b	-	YC	+,	-	.Ns:o]
J09:1060c	-	II	at	at	[P:p.
J09:1060d	-	NN1n	room	room	[Ns.
J09:1060e	-	NN1n	temperature	temperature	.Ns]P:p]
J09:1060f	-	II	on	on	[P:p.
J09:1060g	-	NP1s	Whatman	Whatman	[Ns[Nns.Nns]
J09:1060h	S	FOs	3MM	-	[Nns.Nns]
J09:1060i	-	NN1c	filter	filter	.
J09:1060j	-	NN1n	paper	paper	.Ns]P:p]Tg:h]S]
J09:1060k	-	YF	+.	-	.
J09:1060m	-	MC	Five	five	[S:149[Nup:S.
J09:1070a	-	NNU2	milliamperes	milliampere	.
J09:1070b	-	IIp	+<sol>	-	[P.
J09:1070c	-	NN1c	+cell	cell	.P]Nup:S]
J09:1070d	-	VBDR	were	be	[Vwp.
J09:1070e	-	VVNv	applied	apply	.Vwp]
J09:1070f	-	IF	for	for	[P:t.
J09:1070g	-	MCn	18	-	[Nu.
J09:1070h	S	NNU	hr	-	.Nu]P:t]
J09:1070i	-	YC	+,	-	.
J09:1070j	-	ICSt	after	after	[Fr:x[Pq:t.
J09:1070k	-	DDQr	which	which	[Dq:149.Dq:149]Pq:t]
J09:1070m	-	AT	the	the	[Np:S.
J09:1070n	-	NN2	strips	strip	.Np:S]
J09:1080a	-	VBDR	were	be	[Vwp.
J09:1080b	-	VVNv	stained	stain	.Vwp]
J09:1080c	-	IW	with	with	[P:h.
J09:1080d	-	NN1u	bromphenol	bromphenol	[J.
J09:1080e	-	JJ	blue	blue	.J]P:h]
J09:1080f	-	CC	and	and	[Fr+.
J09:1080g	-	NN1u	densitometry	densitometry	[Ns:S.Ns:S]
J09:1080h	-	VBDZ	was	be	[Vsp.
J09:1080i	-	VVNv	carried	carry	.Vsp]
J09:1080j	-	RP	out	out	[R:n.R:n]
J09:1080k	-	VVGt	using	use	[Tg:h[Vg.Vg]
J09:1090a	-	AT1	a	a	[Nns:o.
J09:1090b	-	NP1j	Spinco	Spinco	.
J09:1090c	-	NN1c	Analytrol	Analytrol	.Nns:o]Tg:h]Fr+]Fr:x]S:149]
J09:1090d	-	YF	+.	-	.O]
J09:1090e	-	YB	<minbrk>	-	[Oh.Oh]
J09:1090f	-	CSn	When	when	[O[S[Fa:t[Rq:t.Rq:t]
J09:1090g	-	NN1n	paper	paper	[Ns:s.
J09:1090h	-	NN1u	electrophoresis	electrophoresis	.Ns:s]
J09:1090i	-	VBDZ	was	be	[Vsb.Vsb]
J09:1090j	-	TO	to	to	[Ti:z[Vip.
J09:1090k	-	VB0	be	be	.
J09:1100a	-	VVNt	used	use	.Vip]
J09:1100b	-	IF	for	for	[P:c.
J09:1100c	-	NN1n	preparation	preparation	.P:c]Ti:z]Fa:t]
J09:1100d	-	YC	+,	-	.
J09:1100e	-	MC	eight	eight	[Np:S.
J09:1100f	-	NN2	strips	strip	.
J09:1100g	-	IO	of	of	[Po.
J09:1100h	-	AT1	a	a	[N.
J09:1100i	-	JJ	whole	whole	[Ns.
J09:1100j	-	NN1u	serum	serum	.Ns]
J09:1100k	-	NN1c	sample	sample	.
J09:1100m	-	CCr	or	or	[Ns+:151.
J09:1100n	-	AT1	a	a	.
J09:1100p	-	JJ	chromatographic	chromatographic	.
J09:1110a	-	NN1c	fraction	fraction	.
J09:1110b	-	YG	-	-	[Tn[S151.S151]
J09:1110c	-	VVNv	concentrated	concentrate	[Vn.Vn]
J09:1110d	-	IIb	by	by	[Pb:h.
J09:1110e	-	JJ	negative	negative	[Ns[Ns.
J09:1110f	-	NN1n	pressure	pressure	.Ns]
J09:1110g	-	NN1u	dialysis	dialysis	.Ns]Pb:h]Tn]Ns+:151]N]Po]Np:S]
J09:1110h	-	VBDR	were	be	[Vwp.
J09:1110i	-	VVNv	run	run	.Vwp]
J09:1110j	-	IIp	+<sol>	-	[P:h.
J09:1110k	-	NNL1c	+chamber	chamber	.P:h]
J09:1120a	-	II	under	under	[P:h.
J09:1120b	-	AT	the	the	[Np:153.
J09:1120c	-	NN2	conditions	condition	.
J09:1120d	-	YG	-	-	[Tn[S153.S153]
J09:1120e	-	VVNt	described	describe	[Vn.Vn]
J09:1120f	-	RL	above	above	[R:p.R:p]Tn]Np:153]P:h]S]
J09:1120g	-	YF	+.	-	.
J09:1120h	-	II	At	at	[S[P:t.
J09:1120i	-	AT	the	the	[Ns.
J09:1120j	-	NN1c	end	end	.
J09:1120k	-	IO	of	of	[Po.
J09:1120m	-	AT	the	the	[Ns.
J09:1120n	-	NN1c	run	run	.Ns]Po]Ns]P:t]
J09:1120p	-	YC	+,	-	.
J09:1130a	-	AT	the	the	[Np:S.
J09:1130b	-	NN2	strips	strip	.
J09:1130c	-	II	in	in	[P.
J09:1130d	-	AT	the	the	[Np.
J09:1130e	-	MD	third	third	[MD&.
J09:1130f	-	CC	and	and	[MD+.
J09:1130g	-	MD	sixth	sixth	.MD+]MD&]
J09:1130h	-	NN2	positions	position	.
J09:1130i	-	II	in	in	[P.
J09:1130j	-	DD1q	each	each	[Ns.
J09:1130k	-	NNL1c	chamber	chamber	.Ns]P]Np]P]Np:S]
J09:1130m	-	VBDR	were	be	[Vwp.
J09:1130n	-	VVNv	dried	dry	.Vwp]
J09:1130p	-	YC	+,	-	.
J09:1140a	-	VVNv	stained	stain	[S-[Vn.Vn]
J09:1140b	-	IF	for	for	[P:t.
J09:1140c	-	MC1n	1	-	[Nu.
J09:1140d	S	NNU	hr	-	.Nu]P:t]S-]
J09:1140e	-	YC	+,	-	.
J09:1140f	-	VVNv	washed	wash	[S-[Vn.Vn]S-]
J09:1140g	-	CC	and	and	[S+.
J09:1140h	-	VVNv	dried	dry	[Vn.Vn]S+]
J09:1140i	-	YC	+,	-	.
J09:1140j	-	CS	while	while	[Fa:t.
J09:1140k	-	AT	the	the	[Np:S.
J09:1140m	-	JBo	other	other	.
J09:1140n	-	NN2	strips	strip	.Np:S]
J09:1140p	-	VBDR	were	be	[Vwp.
J09:1150a	-	VVNt	maintained	maintain	.Vwp]
J09:1150b	-	II	in	in	[P:p.
J09:1150c	-	AT1	a	a	[Ns.
J09:1150d	-	JJ	horizontal	horizontal	.
J09:1150e	-	NN1n	position	position	.Ns]P:p]
J09:1150f	-	II	at	at	[P:p.
J09:1150g	-	MC1n	1	-	[Nu.
J09:1150h	S	NNU	+<deg>C	-	.Nu]P:p]Fa:t]S]
J09:1150i	-	YF	+.	-	.
J09:1150j	-	AT	The	the	[S[Np:S.
J09:1150k	-	JJ	unstained	unstained	.
J09:1150m	-	NN2	strips	strip	.Np:S]
J09:1150n	-	YG	-	-	[t155.t155]
J09:1151a	-	VBDR	were	be	[Vwp.
J09:1160a	-	RTn	then	then	[Rsw:G155.Rsw:G155]
J09:1160b	-	VVNt	marked	mark	.Vwp]
J09:1160c	-	YC	+,	-	.
J09:1160d	-	VVGt	using	use	[Tg:b[Vg.Vg]
J09:1160e	-	AT	the	the	[Np:o.
J09:1160f	-	VVNv	stained	stain	[Tn[Vn.Vn]Tn]
J09:1160g	-	MC2	ones	one	.Np:o]
J09:1160h	-	IIa	as	as	[P:j.
J09:1160i	-	AT1	a	a	[Ns.
J09:1160j	-	NN1c	guide	guide	.Ns]P:j]Tg:b]
J09:1160k	-	YC	+,	-	.
J09:1160m	-	CC	and	and	[S+.
J09:1160n	-	VVNv	cut	cut	[Vn.Vn]
J09:1160p	-	RR	transversely	transversely	[R:h.R:h]
J09:1170a	-	RRz	so	so	[R:c.
J09:1170b	-	CSA	as	as	[A.
J09:1170c	-	TO	to	to	[Ti:z[Vi.
J09:1170d	-	VV0v	separate	separate	.Vi]
J09:1170e	-	AT	the	the	[Np:o.
J09:1170f	-	JJ	various	various	.
J09:1170g	-	NN1n	protein	protein	.
J09:1170h	-	NN2	bands	band	.Np:o]Ti:z]A]R:c]S+]S]
J09:1170i	-	YF	+.	-	.
J09:1170j	-	AT	The	the	[S[Np:S157.
J09:1170k	-	NN1c	strip	strip	.
J09:1170m	-	NN2	sections	section	.
J09:1170n	-	YG	-	-	[Tg[s157.s157]
J09:1170p	-	VVGt	containing	contain	[Vg.Vg]
J09:1180a	-	AT1	a	a	[Ns:o.
J09:1180b	-	VVNv	given	give	[Tn[Vn.Vn]Tn]
J09:1180c	-	NN1n	protein	protein	.Ns:o]Tg]Np:S157]
J09:1180d	-	VBDR	were	be	[Vwp.
J09:1180e	-	VVNt	pooled	pool	.Vwp]
J09:1180f	-	YC	+,	-	.
J09:1180g	-	VVNt	eluted	elute	[S-[Vn.Vn]
J09:1180h	-	IW	with	with	[P:h.
J09:1180i	-	MCd	0.5	-	[Nu.
J09:1180j	S	NNU	ml	-	.
J09:1180k	-	IO	of	of	[Po.
J09:1180m	-	JJ	isotonic	isotonic	[Ns.
J09:1190a	-	NN1u	saline	saline	.Ns]Po]Nu]P:h]S-]
J09:1190b	-	YC	+,	-	.
J09:1190c	-	CC	and	and	[S+.
J09:1190d	-	AT	the	the	[Np:S.
J09:1190e	-	NN2	eluates	eluate	.Np:S]
J09:1190f	-	VBDR	were	be	[Vwp.
J09:1190g	-	VVNt	tested	test	.Vwp]
J09:1190h	-	IF	for	for	[P:r.
J09:1190i	-	NN1c	antibody	antibody	[Ns.
J09:1190j	-	NN1n	activity	activity	.Ns]P:r]S+]S]
J09:1190k	-	YF	+.	-	.O]
J09:1190m	-	YBL	<bminhd>	-	[Oh.
J09:1190n	-	NN1u	ULTRACENTRIFUGATION	ultracentrifugation	.
J09:1190p	-	YF	+.	-	.
J09:1190q	-	YBR	<eminhd>	-	.Oh]
J09:1200a	-	NN2	Fractions	fraction	[O[S[Np:S159.
J09:1200b	-	II	from	from	[P.
J09:1200c	-	AT	the	the	[Ns.
J09:1200d	-	NN1c	column	column	.Ns]P]
J09:1200e	-	DDQr	which	which	[Fr[Dq:s159.Dq:s159]
J09:1200f	-	VBDR	were	be	[Vwb.Vwb]
J09:1200g	-	TO	to	to	[Ti:z[Vip.
J09:1200h	-	VB0	be	be	.
J09:1200i	-	VVNt	subjected	subject	.Vip]
J09:1210a	-	IIt	to	to	[P:u.
J09:1210b	-	JJ	analytical	analytical	[Ns.
J09:1210c	-	NN1u	ultracentrifugation	ultracentrifugation	.Ns]P:u]Ti:z]Fr]Np:S159]
J09:1210d	-	VBDR	were	be	[Vwp.
J09:1210e	-	VVNv	concentrated	concentrate	.Vwp]
J09:1210f	-	IIb	by	by	[Pb:a.
J09:1210g	-	JJ	negative	negative	[Ns.
J09:1210h	-	NN1n	pressure	pressure	.
J09:1220a	-	NN1u	dialysis	dialysis	.Ns]Pb:a]
J09:1220b	-	CC	and	and	[S+.
J09:1220c	-	VVNt	dialyzed	dialyse	[Vn.Vn]
J09:1220d	-	IF	for	for	[P:t.
J09:1220e	-	MCn	16	-	[Nu.
J09:1220f	S	NNU	hr	-	.Nu]P:t]
J09:1220g	-	II	in	in	[P:p.
J09:1220h	-	AT	the	the	[Ns.
J09:1220i	-	NN1n	cold	cold	.Ns]P:p]
J09:1220j	-	II	against	against	[P:h.
J09:1220k	-	RR21	at	at	[Np[RR=.
J09:1220m	-	RR22	least	least	.RR=]
J09:1220n	-	MCn	500	-	.
J09:1230a	-	NN2	volumes	volume	.
J09:1230b	-	IO	of	of	[Po.
J09:1230c	-	NN1n	phosphate	phosphate	[Ns[J.
J09:1230d	-	YH	+<hyphen>	-	.
J09:1230e	-	JJh	+buffered	buffer	.J]
J09:1230f	-	NN1u	saline	saline	.
J09:1230g	-	YC	+,	-	.
J09:1230h	S	NNUb	pH	-	[Nu@.
J09:1230i	-	MCd	7.2	-	.Nu@]
J09:1230j	-	YC	+,	-	.
J09:1230k	-	JJ	ionic	ionic	[L[Ns:s.
J09:1230m	-	NN1n	strength	strength	.Ns:s]
J09:1230n	-	MCd	0.154	-	[M:e.M:e]L]Ns]Po]Np]P:h]S+]S]
J09:1230p	-	YF	+.	-	.
J09:1240a	-	PPHS2	They	they	[S[Nap:S.Nap:S]
J09:1240b	-	YG	-	-	[t161.t161]
J09:1240c	-	VBDR	were	be	[Vwp.
J09:1240d	-	RTn	then	then	[Rsw:G161.Rsw:G161]
J09:1240e	-	VVNt	centrifuged	centrifuge	.Vwp]
J09:1240f	-	II	at	at	[P:h.
J09:1240g	-	MCn	59,780	-	[Nu.
J09:1240h	S	NNU	rpm	-	.Nu]P:h]
J09:1240i	-	IF	for	for	[P:t.
J09:1240j	-	MCn	35	-	[Nu[M.
J09:1240k	-	IIt	to	to	[P.
J09:1240m	-	MCn	80	-	.P]M]
J09:1240n	S	NNU	min	-	.Nu]P:t]
J09:1250a	-	II	at	at	[P:p.
J09:1250b	-	MCn	20	-	[Nu.
J09:1250c	S	NNU	+<deg>C	-	.Nu]P:p]
J09:1250d	-	II	in	in	[P:p.
J09:1250e	-	AT1	a	a	[Ns.
J09:1250f	-	NP1j	Spinco	Spinco	[Nns.Nns]
J09:1250g	-	NN1c	model	model	[Nns.
J09:1250h	S	ZZ1	E	-	.Nns]
J09:1250i	-	NN1c	ultracentrifuge	ultracentrifuge	.Ns]P:p]
J09:1250j	-	II	at	at	[P:p.
J09:1250k	-	AT1	a	a	[Ns.
J09:1250m	-	NN1n	protein	protein	.
J09:1250n	-	NN1n	concentration	concentration	.
J09:1260a	-	IO	of	of	[Po.
J09:1260b	-	MCd	1.00	-	[Nu[M.
J09:1260c	-	IIt	to	to	[P.
J09:1260d	-	MCd	1.25	-	.P]M]
J09:1260e	-	NNUp	+%	-	.Nu]Po]Ns]P:p]S]
J09:1260f	-	YF	+.	-	.
J09:1260g	-	NN1u	Sedimentation	sedimentation	[S[Np:S.
J09:1260h	-	NN2	coefficients	co<hyphen>efficient	.Np:S]
J09:1260i	-	VBDR	were	be	[Vwp.
J09:1260j	-	VVNv	computed	compute	.Vwp]
J09:1270a	-	IIa	as	as	[P:e.
J09:1270b	-	FO	<formul>	-	[Np.
J09:1270c	-	NN2	values	value	.Np]P:e]
J09:1270d	-	CC	and	and	[S+.
J09:1270e	-	JJ	relative	relative	[Np:S.
J09:1270f	-	NN2	amounts	amount	.
J09:1270g	-	IO	of	of	[Po.
J09:1270h	-	AT	the	the	[Np.
J09:1270i	-	JJ	various	various	.
J09:1270j	-	NN2	components	component	.Np]Po]Np:S]
J09:1270k	-	VBDR	were	be	[Vwp.
J09:1270m	-	VVNv	calculated	calculate	.Vwp]
J09:1280a	-	II	from	from	[P:h.
J09:1280b	-	AT	the	the	[Np.
J09:1280c	-	NP1s	Schlieren	Schlieren	[Nns.Nns]
J09:1280d	-	NN2	patterns	pattern	.Np]P:h]S+]S]
J09:1280e	-	YF	+.	-	.O]
J09:1280f	-	YB	<minbrk>	-	[Oh.Oh]
J09:1280g	-	IF	For	for	[O[S[P:c.
J09:1280h	-	JJ	preparative	preparative	[Ns.
J09:1280i	-	NN1u	ultracentrifugation	ultracentrifugation	.Ns]P:c]
J09:1280j	-	YC	+,	-	.
J09:1290a	-	NN2	fractions	fraction	[Np:S.Np:S]
J09:1290b	-	II	from	from	[P:q.
J09:1290c	-	AT	the	the	[Ns.
J09:1290d	-	NN1c	column	column	.Ns]P:q]
J09:1290e	-	VBDR	were	be	[Vwp.
J09:1290f	-	VVNv	concentrated	concentrate	.Vwp]
J09:1290g	-	IIb	by	by	[Pb:a.
J09:1290h	-	JJ	negative	negative	[Ns.
J09:1290i	-	NN1n	pressure	pressure	.
J09:1300a	-	NN1u	dialysis	dialysis	.Ns]Pb:a]
J09:1300b	-	IIt	to	to	[P:e.
J09:1300c	-	NN2	volumes	volume	[Np.
J09:1300d	-	IO	of	of	[Po.
J09:1300e	-	MC1n	1	-	[Nu.
J09:1300f	S	NNU	ml	-	.
J09:1300g	-	CCr	or	or	[D+.
J09:1300h	-	DAR	less	less	.D+]Nu]Po]Np]P:e]
J09:1300i	-	YC	+,	-	.
J09:1300j	-	VVNv	transferred	transfer	[S-[Vn.Vn]
J09:1300k	-	IIt	to	to	[P:q.
J09:1300m	-	NN1u	cellulose	cellulose	[Np.
J09:1310a	-	NN2	tubes	tube	.Np]P:q]S-]
J09:1310b	-	CC	and	and	[S+.
J09:1310c	-	VVNt	diluted	dilute	[Vn.Vn]
J09:1310d	-	IIt	to	to	[P:e.
J09:1310e	-	MCn	12	-	[Nu.
J09:1310f	S	NNU	ml	-	.Nu]P:e]
J09:1310g	-	IW	with	with	[P:h.
J09:1310h	-	JJ	isotonic	isotonic	[Ns.
J09:1310i	-	NN1u	saline	saline	.Ns]P:h]S+]S]
J09:1310j	-	YF	+.	-	.
J09:1310k	-	NN1u	Ultracentrifugation	ultracentrifugation	[S[Ns:S.Ns:S]
J09:1310m	-	YG	-	-	[t163.t163]
J09:1320a	-	VBDZ	was	be	[Vsp.
J09:1320b	-	RTn	then	then	[Rsw:G163.Rsw:G163]
J09:1320c	-	VVNv	carried	carry	.Vsp]
J09:1320d	-	RP	out	out	[R:n.R:n]
J09:1320e	-	II	in	in	[P:p.
J09:1320f	-	AT1	a	a	[Ns.
J09:1320g	-	NP1j	Spinco	Spinco	[Nns.Nns]
J09:1320h	-	NN1c	model	model	[Nns.
J09:1320i	S	FOs	L	-	.Nns]
J09:1320j	-	NN1c	ultracentrifuge	ultracentrifuge	.Ns]P:p]
J09:1320k	-	II	at	at	[P:h.
J09:1330a	-	MCn	40,000	-	[Nu.
J09:1330b	S	NNU	rpm	-	.Nu]P:h]
J09:1330c	-	IF	for	for	[P:t.
J09:1330d	-	MCn	125	-	[Nu.
J09:1330e	-	IIt	to	to	[P.
J09:1330f	-	MCn	150	-	.P]
J09:1330g	S	NNU	min	-	.Nu]P:t]
J09:1330h	-	YC	+,	-	.
J09:1330i	-	NN1n	refrigeration	refrigeration	[Tg:b[Ns:S.Ns:S]
J09:1330j	-	NN1n	being	being	[Vgp.
J09:1330k	-	VVNt	used	use	.Vgp]
J09:1330m	-	II	throughout	throughout	[P:t.
J09:1340a	-	AT	the	the	[Ns.
J09:1340b	-	NN1c	run	run	.Ns]P:t]Tg:b]S]
J09:1340c	-	YF	+.	-	.
J09:1340d	-	JJ	Successive	successive	[S[Np:S.
J09:1340e	-	MC1n	1	-	[Nu.
J09:1340f	-	YH	+<hyphen>	-	.
J09:1340g	S	NNU	+ml	-	.Nu]
J09:1340h	-	NN2	fractions	fraction	.Np:S]
J09:1340i	-	YG	-	-	[t165.t165]
J09:1340j	-	VBDR	were	be	[Vwp.
J09:1340k	-	RTn	then	then	[Rsw:G165.Rsw:G165]
J09:1340m	-	VVNv	drawn	draw	.Vwp]
J09:1340n	-	RP	off	off	[R:q.R:q]
J09:1340p	-	IW	with	with	[P:h.
J09:1340q	-	AT1	a	a	[Ns.
J09:1350a	-	JJ	hypodermic	hypodermic	.
J09:1350b	-	NN1c	syringe	syringe	.Ns]P:h]
J09:1350c	-	YC	+,	-	.
J09:1350d	-	VVGv	starting	start	[Tg:b[Vg.Vg]
J09:1350e	-	II	at	at	[P:p.
J09:1350f	-	AT	the	the	[Ns.
J09:1350g	-	NN1c	top	top	.
J09:1350h	-	IO	of	of	[Po.
J09:1350i	-	AT	the	the	[Ns.
J09:1350j	-	NN1n	tube	tube	.Ns]Po]Ns]P:p]Tg:b]
J09:1350k	-	YC	+,	-	.
J09:1350m	-	CC	and	and	[S+.
J09:1350n	-	VVNt	tested	test	[Vn.Vn]
J09:1350p	-	IF	for	for	[P:r.
J09:1350q	-	NN1n	agglutinin	agglutinin	[Ns.
J09:1360a	-	NN1n	activity	activity	.Ns]P:r]S+]S]
J09:1360b	-	YF	+.	-	.O]
J09:1360c	-	YB	<minbrk>	-	[Oh.Oh]
J09:1360d	-	JBo	Other	other	[O[S[Np:S.
J09:1360e	-	NN2	methods	method	.Np:S]
J09:1360f	-	VMo	will	will	[Vcp.
J09:1360g	-	VB0	be	be	.
J09:1360h	-	VVNt	described	describe	.Vcp]
J09:1360i	-	RL	below	below	[R:p.R:p]S]
J09:1360j	-	YF	+.	-	.O]
J09:1370a	-	YBL	<bmajhd>	-	[Oh.
J09:1370b	-	JJ	EXPERIMENTAL	experimental	[J.
J09:1370c	-	CC	AND	and	[Np+.
J09:1370d	-	NN2	RESULTS	result	.Np+]J]
J09:1370e	-	YBR	<emajhd>	-	.Oh]
J09:1370f	-	AT	The	the	[O[S[Ns:s167.
J09:1370g	-	JJ	insoluble	insoluble	.
J09:1370h	-	NN1n	material	material	.
J09:1370i	-	DDQr	which	which	[Fr[Dq:s167.Dq:s167]
J09:1370j	-	VVDt	precipitated	precipitate	[Vd.Vd]
J09:1380a	-	II	during	during	[P:t.
J09:1380b	-	NN1u	dialysis	dialysis	.P:t]
J09:1380c	-	II	against	against	[P:h.
J09:1380d	-	VVGv	starting	start	[Ns[Tg[Vg.Vg]Tg]
J09:1380e	-	NN1c	buffer	buffer	.Ns]P:h]Fr]Ns:s167]
J09:1380f	-	RR	always	always	[R:t.R:t]
J09:1380g	-	VVDv	showed	show	[Vd.Vd]
J09:1380h	-	JJ	intense	intense	[Ns:o.
J09:1380i	-	NN1n	agglutinin	agglutinin	.
J09:1390a	-	NN1n	activity	activity	.Ns:o]
J09:1390b	-	YC	+,	-	.
J09:1390c	-	JJ	regardless	regardless	[Jh:c.
J09:1390d	-	IO	of	of	[Po.
J09:1390e	-	AT	the	the	[Ns.
J09:1390f	-	NN1n	blood	blood	.
J09:1390g	-	NNJ1c	group	group	.
J09:1390h	-	IO	of	of	[Po.
J09:1390i	-	AT	the	the	[Ns.
J09:1390j	-	NN1c	donor	donor	.Ns]Po]Ns]Po]Jh:c]S]
J09:1390k	-	YF	+.	-	.
J09:1390m	-	IW	With	with	[S[P:r.
J09:1390n	-	DD1e	either	either	[Ds.
J09:1400a	-	IO	of	of	[Po.
J09:1400b	-	AT	the	the	[Np:169.
J09:1400c	-	NN2	gradients	gradient	.
J09:1400d	-	YG	-	-	[Tn[S169.S169]
J09:1400e	-	VVNt	described	describe	[Vn.Vn]Tn]Np:169]Po]Ds]P:r]
J09:1400f	-	YC	+,	-	.
J09:1400g	-	NN1u	chromatography	chromatography	[Ns:s.
J09:1400h	-	II	on	on	[P.
J09:1400i	S	FOc	DEAE	-	[Ns.
J09:1400j	-	YH	+<hyphen>	-	.
J09:1400k	-	NN1u	+cellulose	cellulose	.Ns]P]Ns:s]
J09:1410a	-	VVDv	separated	separate	[Vd.Vd]
J09:1410b	-	NN2	agglutinins	agglutinins	[Np:o.
J09:1410c	-	IO	of	of	[Po.
J09:1410d	-	AT	the	the	[N.
J09:1410e	S	FOs	ABO	-	.
J09:1410f	-	NNc	series	series	.N]Po]Np:o]
J09:1410g	-	II	into	into	[P:q.
J09:1410h	-	RR21	at	at	[Np[RR=.
J09:1410i	-	RR22	least	least	.RR=]
J09:1410j	-	MC	three	three	.
J09:1410k	-	NN2	regions	region	.
J09:1420a	-	YPL	(	-	.
J09:1420b	A	NNmm	+Figs.	-	[Iu[Nnp.
J09:1420c	-	MC1n	1	-	[MC1n&.
J09:1420d	-	CC	and	and	[MCn+.
J09:1420e	-	MCn	2	-	.MCn+]MC1n&]Nnp]Iu]
J09:1420f	-	YPR	+)	-	.
J09:1420g	-	YN	+:	-	.
J09:1420h	-	MC1	one	one	[Ms@.
J09:1420i	-	IO	of	of	[Po.
J09:1420j	-	RR	extremely	extremely	[Ns[J.
J09:1420k	-	JJ	low	low	.J]
J09:1420m	-	JJ	anionic	anionic	.
J09:1420n	-	VVGv	binding	bind	[Tg[Vg.Vg]Tg]
J09:1420p	-	NN1n	capacity	capacity	.Ns]Po]
J09:1420q	-	YC	+,	-	.
J09:1430a	-	MC1	one	one	[Ms-.
J09:1430b	-	IO	of	of	[Po.
J09:1430c	-	JJ	low	low	[Ns.
J09:1430d	-	JJ	anionic	anionic	.
J09:1430e	-	VVGv	binding	bind	[Tg[Vg.Vg]Tg]
J09:1430f	-	NN1n	capacity	capacity	.Ns]Po]Ms-]
J09:1430g	-	CC	and	and	[Ms+.
J09:1430h	-	MC1	one	one	.
J09:1430i	-	IO	of	of	[Po.
J09:1430j	-	JJ	high	high	[Ns.
J09:1430k	-	JJ	anionic	anionic	.
J09:1430m	-	VVGv	binding	bind	[Tg[Vg.Vg]Tg]
J09:1440a	-	NN1n	capacity	capacity	.Ns]Po]Ms+]Ms@]Np]P:q]S]
J09:1440b	-	YF	+.	-	.
J09:1440c	-	DD2i	These	these	[S[Dp:S.Dp:S]
J09:1440d	-	VH0	have	have	[Vfp.
J09:1440e	-	VBN	been	be	.
J09:1440f	-	VVNt	labeled	label	.Vfp]
J09:1440g	-	NN2	Regions	region	[Nnp:e.
J09:1440h	-	MC1n	1	-	[Ms.
J09:1440i	-	YC	+,	-	.
J09:1440j	-	MCn	2	-	[M-.M-]
J09:1440k	-	YC	+,	-	.
J09:1440m	-	CC	and	and	[M+.
J09:1440n	-	MCn	4	-	.M+]
J09:1440p	-	YC	+,	-	.
J09:1440q	-	RAc	respectively	respectively	.
J09:1440r	-	YC	+,	-	.Ms]Nnp:e]
J09:1450a	-	II	in	in	[P:p.
J09:1450b	A	NNm	Fig.	-	[Nns.
J09:1450c	-	MC1n	1	-	.Nns]P:p]S]
J09:1450d	-	YF	+.	-	.
J09:1450e	-	CSn	When	when	[S[Fa:t[Rq:t.Rq:t]
J09:1450f	-	AT	the	the	[Ns:S.
J09:1450g	-	JJ	early	early	.
J09:1450h	-	NN1c	part	part	.
J09:1450i	-	IO	of	of	[Po.
J09:1450j	-	AT	the	the	[Ns.
J09:1450k	-	NN1c	gradient	gradient	.Ns]Po]Ns:S]
J09:1450m	-	VBDZ	was	be	[Vsp.
J09:1450n	-	VVNv	flattened	flatten	.Vsp]
J09:1450p	-	YC	+,	-	.
J09:1460a	-	LEe	either	either	[Pb:h.
J09:1460b	-	IIb	by	by	.
J09:1460c	-	VVGt	using	use	[Tg[Vg.Vg]
J09:1460d	-	AT	the	the	[Ns:o171.
J09:1460e	-	NN1c	gradient	gradient	.
J09:1460f	-	YG	-	-	[Tn[S171.S171]
J09:1460g	-	VVNv	shown	show	[Vn.Vn]
J09:1460h	-	II	in	in	[P:p.
J09:1460i	A	NNm	Fig.	-	[Nns.
J09:1460j	-	MCn	2	-	.Nns]P:p]Tn]Ns:o171]Tg]
J09:1460k	-	CCr	or	or	[Pb+.
J09:1460m	-	IIb	by	by	.
J09:1460n	-	VVGv	allowing	allow	[Tg[Vg.Vg]
J09:1460p	-	AT	the	the	[Ns:O173.
J09:1460q	-	YIL	<ldquo>	-	.
J09:1460r	-	NN1c	+cone	cone	[N.
J09:1460s	-	YH	+<hyphen>	-	.
J09:1460t	-	NN1c	+sphere	sphere	.N]
J09:1460u	-	YIR	+<rdquo>	-	.
J09:1470a	-	NN1c	gradient	gradient	.Ns:O173]
J09:1470b	-	YG	-	-	[Ti:o[s173.s173]
J09:1470c	-	TO	to	to	[Vi.
J09:1470d	-	VV0v	become	become	.Vi]
J09:1470e	-	VVNt	established	establish	[Tn:e[Vn.Vn]Tn:e]
J09:1470f	-	DAR	more	more	[R:h.
J09:1470g	-	RR	slowly	slowly	.R:h]Ti:o]Tg]Pb+]Pb:h]Fa:t]
J09:1470h	-	YC	+,	-	.
J09:1470i	-	NN1c	Region	region	[Ns:S[Nns.
J09:1470j	-	MCn	2	-	.Nns]
J09:1470k	-	NN1n	activity	activity	.Ns:S]
J09:1470m	-	YG	-	-	[t175.t175]
J09:1480a	-	VMd	could	can	[Vdcp.
J09:1480b	-	RR	sometimes	sometimes	[R:G175.R:G175]
J09:1480c	-	VB0	be	be	.
J09:1480d	-	VVNv	separated	separate	.Vdcp]
J09:1480e	-	II	into	into	[P:q.
J09:1480f	-	MC	two	two	[Np.
J09:1480g	-	NN2	areas	area	.
J09:1480h	-	YPL	(	-	.
J09:1480i	-	NN2	+donors	donor	[Iu[Nn[Nn.
J09:1480j	A	NP1i	P.	-	.
J09:1480k	A	NP1i	J.	-	.
J09:1490a	-	CC	and	and	[Nns+.
J09:1490b	A	NP1i	R.	-	.
J09:1490c	A	NP1i	S.	-	.Nns+]
J09:1490d	-	YC	+,	-	.
J09:1490e	A	NNm	Fig.	-	[Nns@.
J09:1490f	-	MC1n	1	-	.Nns@]Nn]
J09:1490g	-	CC	and	and	[Nns+.
J09:1490h	A	NP1i	E.	-	.
J09:1490i	A	NP1i	M.	-	.
J09:1490j	-	YC	+,	-	.
J09:1490k	A	NNm	Fig.	-	[Nns@.
J09:1490m	-	MCn	2	-	.Nns@]Nns+]Nn]Iu]
J09:1490n	-	YPR	+)	-	.Np]P:q]S]
J09:1490p	-	YF	+.	-	.
J09:1490q	-	AT	The	the	[S[Ns:s.
J09:1490r	-	DAr	latter	latter	.
J09:1500a	-	NN1n	procedure	procedure	.Ns:s]
J09:1500b	-	VVDv	gave	give	[Vd.Vd]
J09:1500c	-	NNL1c	rise	rise	[Ns:o.Ns:o]
J09:1500d	-	IIt	to	to	[P:u.
J09:1500e	-	AT1	a	a	[Ns.
J09:1500f	-	JJ	small	small	.
J09:1500g	-	JJ	active	active	[Ns.
J09:1500h	-	NN1n	protein	protein	.Ns]
J09:1500i	-	NN1c	peak	peak	.Ns]P:u]
J09:1500j	-	YPL	(	-	.
J09:1500k	-	NN1c	+Region	region	[Iu[Nns.
J09:1500m	-	MCb	1a	-	.Nns]Iu]
J09:1500n	-	YPR	+)	-	.
J09:1500p	-	II	between	between	[P:p.
J09:1510a	-	NN2	Regions	region	[Nnp.
J09:1510b	-	MC1n	1	-	[MC1n&.
J09:1510c	-	CC	and	and	[MCn+.
J09:1510d	-	MCn	2	-	.MCn+]MC1n&]Nnp]P:p]S]
J09:1510e	-	YF	+.	-	.
J09:1510f	-	II	In	in	[S[P:p.
J09:1510g	-	MCn	2	-	[Np[M.
J09:1510h	-	IO	of	of	[Po.
J09:1510i	-	MCn	15	-	.Po]M]
J09:1510j	-	NN2	experiments	experiment	.
J09:1510k	-	II	on	on	[P.
J09:1510m	-	JJ	whole	whole	[Ns.
J09:1510n	-	NN1u	serum	serum	.Ns]P]Np]P:p]
J09:1510p	-	AT1	a	a	[Ns:S.
J09:1510q	-	NN1c	region	region	.
J09:1510r	-	IO	of	of	[Po.
J09:1520a	-	NN1n	agglutinin	agglutinin	[Ns.
J09:1520b	-	NN1n	activity	activity	.
J09:1520c	-	IW	with	with	[P.
J09:1520d	-	JJ	intermediate	intermediate	[Ns.
J09:1520e	-	JJ	anionic	anionic	.
J09:1520f	-	VVGv	binding	bind	[Tg[Vg.Vg]Tg]
J09:1520g	-	NN1n	capacity	capacity	.Ns]P]Ns]Po]Ns:S]
J09:1520h	-	VBDZ	was	be	[Vsp.
J09:1520i	-	VVNt	detected	detect	.Vsp]
J09:1530a	-	YPL	(	-	.
J09:1530b	-	NN1c	+Region	region	[Nns@.
J09:1530c	-	MCn	3	-	.
J09:1530d	-	YC	+,	-	.
J09:1530e	A	NNm	Fig.	-	[Nns@.
J09:1530f	-	MC1n	1	-	.Nns@]Nns@]
J09:1530g	-	YPR	+)	-	.S]
J09:1530h	-	YF	+.	-	.
J09:1530i	-	RR	Moreover	moreover	[S[R:m.R:m]
J09:1530j	-	YC	+,	-	.
J09:1530k	-	ICSt	after	after	[P:t.
J09:1530m	-	NN1n	concentration	concentration	.P:t]
J09:1530n	-	VVGt	using	use	[Tg:h[Vg.Vg]
J09:1530p	-	JJ	negative	negative	[Ns:o[Ns.
J09:1540a	-	NN1n	pressure	pressure	.Ns]
J09:1540b	-	NN1u	dialysis	dialysis	.Ns:o]Tg:h]
J09:1540c	-	YC	+,	-	.
J09:1540d	-	NN1n	agglutinin	agglutinin	[Ns:S.
J09:1540e	-	NN1n	activity	activity	.Ns:S]
J09:1540f	-	YG	-	-	[t177.t177]
J09:1540g	-	VMd	could	can	[Vdcp.
J09:1540h	-	RR	sometimes	sometimes	[R:G177.R:G177]
J09:1540i	-	VB0	be	be	.
J09:1540j	-	VVNt	detected	detect	.Vdcp]
J09:1550a	-	II	in	in	[P:p.
J09:1550b	-	AT	the	the	[Ns:179.
J09:1550c	-	NN1c	region	region	.
J09:1550d	-	YG	-	-	[Tn[S179.S179]
J09:1550e	-	VVNt	designated	designate	[Vn.Vn]
J09:1550f	-	MCb	2a	-	[M:e.M:e]Tn]
J09:1550g	-	YPL	(	-	.
J09:1550h	-	NN2	+donors	donor	[Iu[Nn.
J09:1550i	A	NP1i	P.	-	.
J09:1550j	A	NP1i	J.	-	.
J09:1550k	-	YC	+,	-	.
J09:1550m	A	NP1i	D.	-	[Nns-.
J09:1550n	A	NP1i	A.	-	.Nns-]
J09:1550p	-	YC	+,	-	.
J09:1550q	-	CC	and	and	[Nns+.
J09:1560a	A	NP1i	J.	-	.
J09:1560b	A	NP1i	F.	-	.Nns+]
J09:1560c	-	YC	+,	-	.
J09:1560d	A	NNm	Fig.	-	[Nns@.
J09:1560e	-	MC1n	1	-	.Nns@]Nn]Iu]
J09:1560f	-	YPR	+)	-	.Ns:179]P:p]S]
J09:1560g	-	YF	+.	-	.O]
J09:1560h	-	YB	<minbrk>	-	[Oh.Oh]
J09:1560i	-	XX	Not	not	[O[S.
J09:1560j	-	DBa	all	all	[Np:s.
J09:1560k	-	DD2i	these	these	.
J09:1560m	-	NN2	regions	region	.Np:s]
J09:1560n	-	VVDt	exhibited	exhibit	[Vd.Vd]
J09:1560p	-	JJ	equal	equal	[Ns:o.
J09:1570a	-	VVGt	agglutinating	agglutinate	[Tg[Vg.Vg]Tg]
J09:1570b	-	NN1n	activity	activity	.Ns:o]
J09:1570c	-	YC	+,	-	.
J09:1570d	-	CSA	as	as	[A:x.
J09:1570e	-	VVNt	evidenced	evidence	[Vn.Vn]
J09:1570f	-	IIb	by	by	[Pb:a.
J09:1570g	-	NN1n	titer	titre	[N.
J09:1570h	-	CC	and	and	[Ns+.
J09:1570i	-	AT	the	the	.
J09:1570j	-	NN1u	extent	extent	.
J09:1570k	-	IO	of	of	[Po.
J09:1570m	-	AT	the	the	[Np.
J09:1580a	-	JJ	active	active	.
J09:1580b	-	NN2	areas	area	.Np]Po]Ns+]N]Pb:a]A:x]S]
J09:1580c	-	YF	+.	-	.
J09:1580d	-	II	In	in	[S[P:m.
J09:1580e	-	DBa	all	all	[Np.
J09:1580f	-	NN2	cases	case	.Np]P:m]
J09:1580g	-	YC	+,	-	.
J09:1580h	-	DAT	most	most	[Ds:s.
J09:1580i	-	IO	of	of	[Po.
J09:1580j	-	AT	the	the	[Ns.
J09:1580k	-	NN1n	activity	activity	.Ns]Po]Ds:s]
J09:1580m	-	VVDi	lay	lie	[Vd.Vd]
J09:1580n	-	II	in	in	[P:p.
J09:1580p	-	AT	the	the	[Ns.
J09:1580q	-	NN1c	region	region	.
J09:1580r	-	IO	of	of	[Po.
J09:1590a	-	JJ	high	high	[Ns.
J09:1590b	-	JJ	anionic	anionic	.
J09:1590c	-	VVGv	binding	bind	[Tg[Vg.Vg]Tg]
J09:1590d	-	NN1n	capacity	capacity	.Ns]Po]Ns]P:p]S]
J09:1590e	-	YF	+.	-	.
J09:1590f	-	DD1i	This	this	[S[Ds:s.Ds:s]
J09:1590g	-	VBDZ	was	be	[Vsb.Vsb]
J09:1590h	-	RR	particularly	particularly	[J:e.
J09:1590i	-	JJ	noticeable	noticeable	.J:e]
J09:1590j	-	II	in	in	[P:p.
J09:1590k	-	NNJ1c	group	group	[Np:181[Nn.
J09:1600a	S	ZZ1	A	-	.
J09:1600b	-	CC	and	and	[Nns+.
J09:1600c	-	NNJ1c	group	group	.
J09:1600d	S	ZZ1	B	-	.Nns+]Nn]
J09:1600e	-	NN2	sera	serum	.
J09:1600f	-	YC	+,	-	.
J09:1600g	-	II	in	in	[Fr[Pq:m.
J09:1600h	-	DDQr	which	which	[Nqp:181.
J09:1600i	-	NN2	cases	case	.Nqp:181]Pq:m]
J09:1600j	-	NN1n	activity	activity	[Ns:s.
J09:1600k	-	II	in	in	[P.
J09:1600m	-	NN2	Regions	region	[Nnp.
J09:1600n	-	MC1n	1	-	[MC1n&.
J09:1610a	-	CC	and	and	[MCn+.
J09:1610b	-	MCn	2	-	.MCn+]MC1n&]Nnp]P]Ns:s]
J09:1610c	-	VBDZ	was	be	[Vsb.Vsb]
J09:1610d	-	RR	usually	usually	[R:m.R:m]
J09:1610e	-	XX	not	not	.
J09:1610f	-	JJ	detectable	detectable	[J:e.J:e]
J09:1610g	-	IW	without	without	[P:h.
J09:1610h	-	JJ	prior	prior	[Ns.
J09:1610i	-	NN1n	concentration	concentration	.Ns]P:h]
J09:1610j	-	CC	and	and	[Fr+.
J09:1610k	-	RR	occasionally	occasionally	[R:t.R:t]
J09:1620a	-	VMd	could	can	[Vdcep.
J09:1620b	-	XX	not	not	.
J09:1620c	-	VB0	be	be	.
J09:1620d	-	VVNt	detected	detect	.Vdcep]
J09:1620e	-	RR21	at	at	[R:m[RR=.
J09:1620f	-	RR22	all	all	.RR=]R:m]Fr+]Fr]Np:181]P:p]S]
J09:1620g	-	YF	+.	-	.
J09:1620h	-	EX	There	there	[S.
J09:1620i	-	VVDi	appeared	appear	[Vd.Vd]
J09:1620j	-	TO	to	to	[Ti:s[Vib.
J09:1620k	-	VB0	be	be	.Vib]
J09:1620m	-	ATn	no	no	[Ns:s.
J09:1620n	-	NN1n	difference	difference	.
J09:1630a	-	II	in	in	[P.
J09:1630b	-	AT	the	the	[Ns.
J09:1630c	-	NN1n	distribution	distribution	.
J09:1630d	-	IO	of	of	[Po.
J09:1630e	-	FB	anti	anti<hyphen>	[Ns[J.
J09:1630f	-	YH	+<hyphen>	-	.
J09:1630g	S	ZZ1	+A	-	.
J09:1630h	-	CC	and	and	[J+.
J09:1630i	-	FB	anti	anti<hyphen>	.
J09:1630j	-	YH	+<hyphen>	-	.
J09:1630k	S	ZZ1	+B	-	.J+]J]
J09:1630m	-	NN1n	activity	activity	.Ns]Po]Ns]P]Ns:s]
J09:1630n	-	II	in	in	[P:p.
J09:1630p	-	NNJ1c	group	group	[Ns[Nns.
J09:1640a	S	ZZ1	O	-	.Nns]
J09:1640b	-	NN1u	serum	serum	.Ns]P:p]Ti:s]
J09:1640c	-	YC	+,	-	.
J09:1640d	-	CSg	though	though	[Fa:c.
J09:1640e	-	II	in	in	[P:p.
J09:1640f	-	MC	two	two	[Np.
J09:1640g	-	NNJ1c	group	group	[Nns.
J09:1640h	S	ZZ1	O	-	.Nns]
J09:1640i	-	NN2	donors	donor	.
J09:1640j	-	YPL	(	-	.
J09:1640k	A	NP1i	+J.	-	[Nn@.
J09:1640m	A	NP1i	F.	-	.
J09:1640n	-	CC	and	and	[Nns+.
J09:1640p	A	NP1i	E.	-	.
J09:1650a	A	NP1i	M.	-	.Nns+]Nn@]
J09:1650b	-	YPR	+)	-	.Np]P:p]
J09:1650c	-	RRx	only	only	[R:m.R:m]
J09:1650d	-	MC1	one	one	[Ns:S.
J09:1650e	-	NN1n	type	type	.
J09:1650f	-	IO	of	of	[Po.
J09:1650g	-	NN1n	agglutinin	agglutinin	.Po]Ns:S]
J09:1650h	-	VBDZ	was	be	[Vsp.
J09:1650i	-	VVNt	found	find	.Vsp]
J09:1650j	-	II	in	in	[P:p.
J09:1650k	-	AT	the	the	[Np.
J09:1650m	-	NN2	regions	region	.
J09:1650n	-	IO	of	of	[Po.
J09:1650p	-	JJ	low	low	[Ns.
J09:1650q	-	JJ	anionic	anionic	.
J09:1660a	-	VVGv	binding	bind	[Tg[Vg.Vg]Tg]
J09:1660b	-	NN1n	capacity	capacity	.Ns]Po]Np]P:p]
J09:1660c	-	YPL	(	-	.
J09:1660d	A	NNmm	+Figs.	-	[Iu[Nnp.
J09:1660e	-	MC1n	1	-	[MC1n&.
J09:1660f	-	CC	and	and	[MCn+.
J09:1660g	-	MCn	2	-	.MCn+]MC1n&]Nnp]Iu]
J09:1660h	-	YPR	+)	-	.Fa:c]S]
J09:1660i	-	YF	+.	-	.O]
J09:1660j	-	YB	<minbrk>	-	[Oh.Oh]
J09:1660k	-	DA2q	Several	several	[O[S[Np:S.
J09:1660m	-	NN2	samples	sample	.
J09:1660n	-	IO	of	of	[Po.
J09:1670a	-	VVNt	citrated	citrate	[Ns[Tn[Vn.Vn]Tn]
J09:1670b	-	NN1u	plasma	plasma	.Ns]Po]Np:S]
J09:1670c	-	VBDR	were	be	[Vwp.
J09:1670d	-	VVNt	fractionated	fractionate	.Vwp]
J09:1670e	-	II	in	in	[P:p.
J09:1670f	-	APPGi2	our	our	[Ns.
J09:1670g	-	NN1c	laboratory	laboratory	.Ns]P:p]
J09:1670h	-	IIb	by	by	[Pb:h.
J09:1670i	-	NN1n	Method	method	[Nns.
J09:1670j	-	MCn	6	-	.
J09:1670k	-	IO	of	of	[Po.
J09:1670m	-	NP1s	Cohn	Cohn	[Nns.
J09:1680a	-	YTL	<bital>	-	.
J09:1680b	-	RAc21	et	et	[RAc=.
J09:1680c	-	RAc22	al	al	.RAc=]
J09:1680d	-	YTR	<eital>	-	.Nns]Po]Nns]Pb:h]S]
J09:1680e	-	YF	+.	-	.
J09:1680f	-	DD2i	These	these	[S[Np:S.
J09:1680g	-	NN2	fractions	fraction	.Np:S]
J09:1680h	-	VBDR	were	be	[Vwp.
J09:1680i	-	VVNt	tested	test	.Vwp]
J09:1680j	-	IF	for	for	[P:r.
J09:1680k	S	FOs	ABO	-	[Ns.
J09:1680m	-	NN1n	agglutinin	agglutinin	.
J09:1690a	-	NN1n	activity	activity	.Ns]P:r]
J09:1690b	-	YC	+,	-	.
J09:1690c	-	VVGt	using	use	[Tg:b[Vg.Vg]
J09:1690d	-	NN2	fractions	fraction	[Np:o.
J09:1690e	-	II	from	from	[P.
J09:1690f	-	NNJ1c	group	group	[Ns[Nns.
J09:1690g	S	FOs	AB	-	.Nns]
J09:1690h	-	NN1u	plasma	plasma	.Ns]P]Np:o]
J09:1690i	-	IIa	as	as	[P:j.
J09:1690j	-	AT1	a	a	[Ns.
J09:1690k	-	NN1n	control	control	.Ns]P:j]Tg:b]S]
J09:1690m	-	YF	+.	-	.
J09:1700a	-	CSA	As	as	[S[A:x.
J09:1700b	-	VVNt	expected	expect	[Vn.Vn]A:x]
J09:1700c	-	YC	+,	-	.
J09:1700d	-	DAT	most	most	[Ds:S.
J09:1700e	-	IO	of	of	[Po.
J09:1700f	-	AT	the	the	[Ns.
J09:1700g	-	NN1n	activity	activity	.Ns]Po]Ds:S]
J09:1700h	-	VBDZ	was	be	[Vsp.
J09:1700i	-	VVNt	found	find	.Vsp]
J09:1700j	-	II	in	in	[P:p.
J09:1700k	-	NN1c	Fraction	fraction	[Nns.
J09:1700m	-	FO	<formul>	-	.Nns]P:p]
J09:1700n	-	YC	+,	-	.
J09:1700p	-	IW	with	with	[W:b.
J09:1710a	-	JJ	slight	slight	[Ns:S.
J09:1710b	-	NN1n	activity	activity	.Ns:S]
J09:1710c	-	VVNv	seen	see	[Vn.Vn]
J09:1710d	-	II	in	in	[P:p.
J09:1710e	-	NN1c	Fraction	fraction	[Nns.
J09:1710f	-	MCr	IV	-	.
J09:1710g	-	YH	+<hyphen>	-	.
J09:1710h	-	MC1n	+1	-	.Nns]P:p]W:b]S]
J09:1710i	-	YF	+.	-	.
J09:1710j	-	AT1	A	a	[S[Ns:S.
J09:1710k	-	NN1c	sample	sample	.
J09:1710m	-	IO	of	of	[Po.
J09:1710n	-	NN1c	Fraction	fraction	[Nns.
J09:1710p	-	FO	<formul>	-	.Nns]Po]
J09:1720a	-	II	from	from	[P.
J09:1720b	-	NNJ1c	group	group	[Ns[Nns.
J09:1720c	S	ZZ1	O	-	.Nns]
J09:1720d	-	NN1u	plasma	plasma	.Ns]P]Ns:S]
J09:1720e	-	VBDZ	was	be	[Vsp.
J09:1720f	-	VVNv	dissolved	dissolve	.Vsp]
J09:1720g	-	II	in	in	[P:p.
J09:1720h	-	VVGv	starting	start	[Ns[Tg[Vg.Vg]Tg]
J09:1720i	-	NN1c	buffer	buffer	.Ns]P:p]
J09:1720j	-	YC	+,	-	.
J09:1720k	-	VVNt	dialyzed	dialyse	[S-[Vn.Vn]
J09:1720m	-	II	against	against	[P:h.
J09:1730a	-	DD1i	this	this	[Ns.
J09:1730b	-	NN1c	buffer	buffer	.Ns]P:h]S-]
J09:1730c	-	CC	and	and	[S+.
J09:1730d	-	VVNt	subjected	subject	[Vn.Vn]
J09:1730e	-	IIt	to	to	[P:u.
J09:1730f	-	NN1u	chromatography	chromatography	.P:u]
J09:1730g	-	VVGt	using	use	[Tg:h[Vg.Vg]
J09:1730h	-	AT	the	the	[Ns:o183.
J09:1730i	-	NN1c	gradient	gradient	.
J09:1730j	-	YG	-	-	[Tn[S183.S183]
J09:1730k	-	VVNv	shown	show	[Vn.Vn]
J09:1740a	-	II	in	in	[P:p.
J09:1740b	A	NNm	Fig.	-	[Nns.
J09:1740c	-	MCn	2	-	.Nns]P:p]Tn]Ns:o183]Tg:h]S+]S]
J09:1740d	-	YF	+.	-	.
J09:1740e	-	RR21	Once	once	[S[R:t[RR=.
J09:1740f	-	RR22	again	again	.RR=]R:t]
J09:1740g	-	YC	+,	-	.
J09:1740h	-	LE	both	both	[Np:S[J.
J09:1740i	-	FB	anti	anti<hyphen>	.
J09:1740j	-	YH	+<hyphen>	-	.
J09:1740k	S	ZZ1	+A	-	.
J09:1740m	-	CC	and	and	[J+.
J09:1740n	-	FB	anti	anti<hyphen>	.
J09:1740p	-	YH	+<hyphen>	-	.
J09:1740q	S	ZZ1	+B	-	.J+]J]
J09:1740r	-	NN2	activities	activity	.Np:S]
J09:1750a	-	VBDR	were	be	[Vwp.
J09:1750b	-	VVNt	found	find	.Vwp]
J09:1750c	-	II	in	in	[P:p.
J09:1750d	-	AT	the	the	[Ns:185.
J09:1750e	-	JJ	insoluble	insoluble	.
J09:1750f	-	NN1n	material	material	.
J09:1750g	-	YG	-	-	[Tn[S185.S185]
J09:1750h	-	VVNt	precipitated	precipitate	[Vn.Vn]
J09:1750i	-	II	during	during	[P:t.
J09:1750j	-	NN1u	dialysis	dialysis	.P:t]Tn]Ns:185]P:p]S]
J09:1750k	-	YF	+.	-	.
J09:1760a	-	RR	Similarly	similarly	[S[R:h.R:h]
J09:1760b	-	YC	+,	-	.
J09:1760c	-	DB2	both	both	[Np:S.
J09:1760d	-	NN2	types	type	.
J09:1760e	-	IO	of	of	[Po.
J09:1760f	-	NN2	antibodies	antibody	.Po]Np:S]
J09:1760g	-	VBDR	were	be	[Vwp.
J09:1760h	-	VVNt	found	find	.Vwp]
J09:1760i	-	II	in	in	[P:p.
J09:1760j	-	MC	three	three	[Np:187.
J09:1760k	-	NN2	regions	region	.
J09:1760m	-	IO	of	of	[Po.
J09:1760n	-	AT	the	the	[Ns.
J09:1770a	-	JJ	chromatographic	chromatographic	.
J09:1770b	-	NN1n	eluate	eluate	.Ns]Po]
J09:1770c	-	YC	+,	-	.
J09:1770d	-	YG	-	-	[Tg[s187.s187]
J09:1770e	-	VHG	having	have	[Vg.Vg]
J09:1770f	-	RR	extremely	extremely	[Ns:o[J.
J09:1770g	-	JJ	low	low	.
J09:1770h	-	YC	+,	-	.
J09:1770i	-	JJ	low	low	[J-.J-]
J09:1770j	-	YC	+,	-	.
J09:1770k	-	CC	and	and	[J+.
J09:1770m	-	JJ	high	high	.J+]J]
J09:1770n	-	JJ	anionic	anionic	.
J09:1780a	-	VVGv	binding	bind	[Tg[Vg.Vg]Tg]
J09:1780b	-	NN1n	capacity	capacity	.Ns:o]
J09:1780c	-	YC	+,	-	.
J09:1780d	-	RAc	respectively	respectively	[R:m.R:m]
J09:1780e	-	YPL	(	-	.
J09:1780f	A	NNm	+Fig.	-	[Iu[Nns.
J09:1780g	-	MCn	3	-	.Nns]Iu]
J09:1780h	-	YPR	+)	-	.Tg]Np:187]P:p]S]
J09:1780i	-	YF	+.	-	.O]
J09:1780j	-	YB	<minbrk>	-	[Oh.Oh]
J09:1780k	-	NN1u	Chromatography	chromatography	[O[S[Ns:s.
J09:1780m	-	IO	of	of	[Po.
J09:1790a	-	JJ	whole	whole	[Np.
J09:1790b	-	NN2	sera	serum	.Np]Po]Ns:s]
J09:1790c	-	VVDt	revealed	reveal	[Vd.Vd]
J09:1790d	-	CST	that	that	[Fn:o.
J09:1790e	-	AT	the	the	[Np:s.
J09:1790f	-	NN2	areas	area	.
J09:1790g	-	IO	of	of	[Po.
J09:1790h	S	FWs	Rh	-	[Ns[Nn.Nn]
J09:1790i	-	NN1c	antibody	antibody	.
J09:1790j	-	NN1n	activity	activity	.Ns]Po]Np:s]
J09:1790k	-	VBDR	were	be	[Vwb.Vwb]
J09:1790m	-	RR	generally	generally	[J:e.
J09:1800a	-	JJ	continuous	continuous	[JJ&.
J09:1800b	-	CC	and	and	[JJ+.
J09:1800c	-	JJ	wide	wide	.JJ+]JJ&]J:e]Fn:o]S]
J09:1800d	-	YF	+.	-	.
J09:1800e	-	AT	The	the	[S[Ns:s.
J09:1800f	-	JJ	incomplete	incomplete	.
J09:1800g	-	NN1c	antibody	antibody	.
J09:1800h	-	NN1n	activity	activity	.Ns:s]
J09:1800i	-	VVDi	appeared	appear	[Vd.Vd]
J09:1810a	-	II	in	in	[P:p.
J09:1810b	-	AT	the	the	[Ns.
J09:1810c	-	JJ	early	early	.
J09:1810d	-	NN1c	part	part	.
J09:1810e	-	IO	of	of	[Po.
J09:1810f	-	AT	the	the	[Ns.
J09:1810g	-	NN1c	chromatogram	chromatogram	.Ns]Po]Ns]P:p]
J09:1810h	-	YS	+;	-	.
J09:1810i	-	AT	the	the	[S-[Nj:s.
J09:1810j	-	JJ	complete	complete	.Nj:s]
J09:1810k	-	YC	+,	-	.
J09:1810m	-	II	in	in	[P:p.
J09:1810n	-	AT	the	the	[Ns.
J09:1810p	-	DAr	latter	latter	.
J09:1820a	-	NN1c	part	part	.Ns]P:p]S-]S]
J09:1820b	-	YF	+.	-	.
J09:1820c	-	AT	The	the	[S[Ns:s189.
J09:1820d	-	NN1u	serum	serum	.
J09:1820e	-	YG	-	-	[Tg[s189.s189]
J09:1820f	-	VVGt	containing	contain	[Vg.Vg]
J09:1820g	-	AT	the	the	[Ns:o.
J09:1820h	-	JJ	mixed	mixed	.
J09:1820i	-	NN1n	type	type	.
J09:1820j	-	IO	of	of	[Po.
J09:1820k	-	JJ	complete	complete	[Np[JJ&.
J09:1820m	-	CC	and	and	[JJ+.
J09:1820n	-	JJ	incomplete	incomplete	.JJ+]JJ&]
J09:1830a	-	NN2	antibodies	antibody	.Np]Po]Ns:o]Tg]Ns:s189]
J09:1830b	-	VVDv	showed	show	[Vd.Vd]
J09:1830c	-	NN1n	activity	activity	[Ns:o.Ns:o]
J09:1830d	-	II	in	in	[P:p.
J09:1830e	-	DB2	both	both	[Np.
J09:1830f	-	NN2	regions	region	.Np]P:p]
J09:1830g	-	YPL	(	-	.
J09:1830h	A	NNm	+Fig.	-	[Iu[Nns.
J09:1830i	-	MC1n	1	-	.Nns]Iu]
J09:1830j	-	YPR	+)	-	.S]
J09:1830k	-	YF	+.	-	.
J09:1830m	-	II	In	in	[S[P:m.
J09:1830n	-	DBa	all	all	[Np.
J09:1830p	-	NN2	cases	case	.Np]P:m]
J09:1840a	-	AT	the	the	[Ns:S.
J09:1840b	-	NN1n	activity	activity	.
J09:1840c	-	II	against	against	[P.
J09:1840d	-	FO	<formul>	-	[Np.
J09:1840e	-	NN2	cells	cell	.Np]P]Ns:S]
J09:1840f	-	VBDZ	was	be	[Vsp.
J09:1840g	-	VVNv	spread	spread	.Vsp]
J09:1840h	-	II	over	over	[P:p.
J09:1840i	-	AT1	a	a	[Ns.
J09:1840j	-	JJR	wider	wide	[Jh.
J09:1840k	-	YG	-	-	[190.190]Jh]
J09:1840m	-	NN1n	area	area	.
J09:1840n	-	CSN	than	than	[P:190.
J09:1840p	-	DD1a	that	that	[Ds.
J09:1850a	-	IW	with	with	[P.
J09:1850b	-	FO	<formul>	-	[Np.
J09:1850c	-	NN2	cells	cell	.Np]P]Ds]P:190]Ns]P:p]
J09:1850d	-	YC	+,	-	.
J09:1850e	-	JJ	regardless	regardless	[Jh:c.
J09:1850f	-	IO	of	of	[Po.
J09:1850g	-	AT	the	the	[Ns:191.
J09:1850h	-	NN1n	type	type	.
J09:1850i	-	IO	of	of	[Po.
J09:1850j	-	NN1c	test	test	.Po]
J09:1850k	-	YPL	(	-	.
J09:1850m	-	NN1u	+saline	saline	[N@.
J09:1850n	-	YC	+,	-	.
J09:1850p	-	NN1u	albumin	albumin	[Ns-.Ns-]
J09:1850q	-	YC	+,	-	.
J09:1850r	-	JJ	indirect	indirect	[Nns-.
J09:1860a	-	NP1s	Coombs	Coombs	.Nns-]N@]
J09:1860b	-	YPR	+)	-	.
J09:1860c	-	YG	-	-	[Tn[S191.S191]
J09:1860d	-	VVNt	used	use	[Vn.Vn]
J09:1860e	-	IF	for	for	[P:c.
J09:1860f	-	NN1n	comparison	comparison	.P:c]Tn]Ns:191]Po]Jh:c]S]
J09:1860g	-	YF	+.	-	.
J09:1860h	-	AT	The	the	[S[Ns:s193.
J09:1860i	-	JJ	insoluble	insoluble	.
J09:1860j	-	NN1n	material	material	.
J09:1860k	-	YG	-	-	[Tg[s193.s193]
J09:1860m	-	VVGi	resulting	result	[Vg.Vg]
J09:1870a	-	II	from	from	[P:q.
J09:1870b	-	NN1u	dialysis	dialysis	[Ns.
J09:1870c	-	II	against	against	[P.
J09:1870d	-	VVGv	starting	start	[Ns[Tg[Vg.Vg]Tg]
J09:1870e	-	NN1c	buffer	buffer	.Ns]P]Ns]P:q]Tg]Ns:s193]
J09:1870f	-	RR	always	always	[R:t.R:t]
J09:1870g	-	VVDv	showed	show	[Vd.Vd]
J09:1870h	-	JJ	strong	strong	[Ns:o.
J09:1870i	-	NN1n	activity	activity	.Ns:o]S]
J09:1870j	-	YF	+.	-	.
J09:1870k	-	RR21	In	in	[S[R:m[RR=.
J09:1880a	-	RR22	fact	fact	.RR=]R:m]
J09:1880b	-	NN1u	agglutination	agglutination	[Ns:S.
J09:1880c	-	IO	of	of	[Po.
J09:1880d	-	FO	<formul>	-	[Np.
J09:1880e	-	NN2	cells	cell	.Np]Po]
J09:1880f	-	II	in	in	[P.
J09:1880g	-	NN1u	saline	saline	.P]Ns:S]
J09:1880h	-	VMd	could	can	[Vdcp.
J09:1880i	-	VB0	be	be	.
J09:1880j	-	VVNv	produced	produce	.Vdcp]
J09:1880k	-	IIb	by	by	[Pb:h.
J09:1880m	-	AT	the	the	[Ns:195.
J09:1890a	-	JJ	insoluble	insoluble	.
J09:1890b	-	NN1n	material	material	.
J09:1890c	-	II	from	from	[P.
J09:1890d	-	NN2	sera	serum	.P]
J09:1890e	-	YG	-	-	[Tg[s195.s195]
J09:1890f	-	VVGt	containing	contain	[Vg.Vg]
J09:1890g	-	YIL	<ldquo>	-	.
J09:1890h	-	RRx	+only	only	[R:m.R:m]
J09:1890i	-	YIR	+<rdquo>	-	.
J09:1890j	-	JJ	incomplete	incomplete	[Ns:o.
J09:1890k	-	NN1c	antibody	antibody	.
J09:1900a	-	NN1n	activity	activity	.Ns:o]Tg]Ns:195]Pb:h]S]
J09:1900b	-	YF	+.	-	.
J09:1900c	-	DD1i	This	this	[S[Ds:S197.Ds:S197]
J09:1900d	-	YG	-	-	[t199.t199]
J09:1900e	-	VBDZ	was	be	[Vsp.
J09:1900f	-	RRR	later	late	[R:G199.R:G199]
J09:1900g	-	VVNv	known	know	.Vsp]
J09:1900h	-	YG	-	-	[Ti:o[s197.s197]
J09:1900i	-	TO	to	to	[Vib.
J09:1900j	-	VB0	be	be	.Vib]
J09:1900k	-	AT	the	the	[Ns:e.
J09:1900m	-	NN1n	result	result	.
J09:1900n	-	IO	of	of	[Po.
J09:1900p	-	VVGv	concentrating	concentrate	[Tg[Vg.Vg]
J09:1900q	-	AT	the	the	[Ns:o201.
J09:1910a	-	JJ	minute	minute	.
J09:1910b	-	NN1c	amount	amount	.
J09:1910c	-	IO	of	of	[Po.
J09:1910d	-	JJ	complete	complete	[Ns.
J09:1910e	-	NN1c	antibody	antibody	.Ns]Po]
J09:1910f	-	YG	-	-	[Tn[S201.S201]
J09:1910g	-	VVNt	found	find	[Vn.Vn]
J09:1910h	-	II	in	in	[P:p.
J09:1910i	-	DD2i	these	these	[Np.
J09:1910j	-	NN2	sera	serum	.Np]P:p]Tn]Ns:o201]Tg]Po]Ns:e]Ti:o]
J09:1910k	-	YS	+;	-	.
J09:1910m	-	CSn	when	when	[S-[Fa:t[Rq:t.Rq:t]
J09:1910n	-	AT	the	the	[Ns:S.
J09:1910p	-	JJ	insoluble	insoluble	.
J09:1920a	-	NN1c	fraction	fraction	.Ns:S]
J09:1920b	-	VBDZ	was	be	[Vsp.
J09:1920c	-	VVNt	suspended	suspend	.Vsp]
J09:1920d	-	II	in	in	[P:p.
J09:1920e	-	AT1	a	a	[Ns.
J09:1920f	-	NN1n	volume	volume	.
J09:1920g	-	IO	of	of	[Po.
J09:1920h	-	NN1u	saline	saline	[Ns.Ns]Po]
J09:1920i	-	JJ	equal	equal	[Jh.
J09:1920j	-	IIt	to	to	[P.
J09:1920k	-	DD1a	that	that	[Ds.
J09:1920m	-	IO	of	of	[Po.
J09:1930a	-	AT	the	the	[Ns.
J09:1930b	-	JJ	original	original	.
J09:1930c	-	NN1u	serum	serum	.
J09:1930d	-	NN1c	sample	sample	.Ns]Po]Ds]P]Jh]Ns]P:p]Fa:t]
J09:1930e	-	YC	+,	-	.
J09:1930f	-	ATn	no	no	[Ns:S.
J09:1930g	-	JJ	complete	complete	.
J09:1930h	-	NN1c	antibody	antibody	.
J09:1930i	-	NN1n	activity	activity	.Ns:S]
J09:1930j	-	VMd	could	can	[Vdcp.
J09:1930k	-	VB0	be	be	.
J09:1930m	-	VVNt	detected	detect	.Vdcp]S-]S]
J09:1930n	-	YF	+.	-	.O]
